慢性前列腺炎患者5种支原体检测

Detection of five Kinds of Mycoplasmas in Prostatic Fluid of Chronic Idiopathic Prostatitis

目的 了解慢性前列腺炎与 5种支原体感染的相关性。方法 采用巢式聚合酶链反应法 (nPCR)检测慢性前列腺炎 (CIP)患者前列腺液中UU、MG、MPN、MH和MF 5种支原体。结果 48例CIP中 3 1例支原体阳性 ,阳性率64 .6% ;其中UU阳性 2 0例、MG阳性 5例、MPN阳性 5例、MH阳性 4例、MF阳性 3例 ;UU +MG阳性 2例 ,UU +MH阳性 1例 ,MPN +MF阳性 1例 ,UU +MG +MPN阳性 1例。结论 支原体感染是CIP的重要致病原因 ,应当引起重视 ;用 1种通用引物查 5种支原体有较大的临床应用价值。

Objective To study the relation of chronic idiopathic prostatitis(CIP) with five kinds of mycoplasmas.Methods UU,MG,MPN,MH,and MF in prostatic fluid of CIP patients were detected by nest PCR.Results 31 of 48 patients were positive,the total positive ratio of five kinds of mycoplasmas was 64.6%(UU positive 20 cases,MG positive 5 cases,MPN positive 5 cases,MH positive 4 cases,MF positive 3 cases;UU+MG positive 2 cases,UU+MH positive 1 cases,MPN+MF positive 1 cases,UU+MG+MPN positive 1 cases).Conclusion The infection of mycoplasmas is important cause of CIP;using a paires of primer to detect the five kinds of mycoplasmas will be a good clinical method.