摘要
目的 :为阐明及防治烧伤后负氮平衡提供理论依据。方法 :在接近机体内环境下 ,于体外分别用免疫沉淀扣除法和间接ELISA法检测IL 1对 30 %体表皮肤全层烫伤大鼠烧伤后骨骼肌中 2 6S蛋白酶复合体及 1 9S调节复合体的活性和蛋白水平的影响。结果 :IL 1可以刺激烧伤后大鼠骨骼肌中 2 6S蛋白酶复合体及 1 9S调节复合体的活性和蛋白表达水平明显升高。结论 :IL 1对烧伤后大鼠骨骼肌中 2 6S蛋白酶复合体系统的激活具有重要作用 ,从而加强蛋白质的分解代谢 。
Try to clarificate the mechanism of negative nitrogen balance after burns.Methods: The means of immuno precipitation deduction and ELISA were used to test the change of activities and contents of 26S proteasome and 19S regulator in skeletal muscle of rats inflicted with 30% TBSAⅢ°burns.Results: IL 1 can markedly raised the activities and contents of 26S proteasome and 19S reguator in rats skeletal muscle after scalded.Conclusions: IL 1 can activate the 26S proteasome system in skeletal muscle,thus enhance the degradation of protein,which is associated with the development of negative nitrogen balance following scald.
出处
《中国烧伤创疡杂志》
2002年第2期81-84,共4页
The Chinese Journal of Burns Wounds & Surface Ulcers
