当归醇沉物及其中性组份体外对巨噬细胞分泌TNF-α和IL-1的影响

Effects of ethanol sediments from tuber of Angelica sinensis and its litmusless component on secreting TNF-α and IL-1 of macrophages in vitro

目的 观察当归醇沉物 (ESA)及其中性组分 (ESA- 1)体外对小鼠腹腔巨噬细胞分泌 TNF- α、IL- 1的影响。方法 L 92 9细胞株细胞毒法测 TNF-α;L 92 9细胞增殖法测 IL - 1。结果 ESA、ESA- 1与小鼠腹腔巨噬细胞(MΦ)共同培养可显著增强其 TNF- α、IL- 1的分泌。在 5～ 2 0 μg/ m L 浓度范围 ,ESA的这种作用呈剂量依赖性 ;ESA- 1虽有明显的作用 ,但未表现出剂量依赖。结论 当归醇沉物及其中性组分体外可增强小鼠腹腔巨噬细胞分泌 TNF-α、IL - 1。

Object To investigate the effects of ethanol sediments obtained from the tuber of Angelica sinensis (Oliv). Diels (ESA) and its litmusless component (ESA 1) on the secretion of TNF α and IL 1 by mice peritoneal macrophages in vitro. Methods L929 cell line cytotoxicity was used for the assay of TNF α. The proliferation of L929 cell line was used for the assay of IL 1. Results The secretion of TNF α and IL 1 by mice peritoneal macrophages which were co cultured with ESA or ESA 1 in vitro can be significantly promoted. At the concentrations in range of 5～20 μg/mL, there is a dose dependence in the action of ESA, while there is not the similar effect of ESA 1, even though it showed the marked effect. Conclusion ESA and ESA 1 can enhance the secreting TNF α and IL 1 of mice peritoneal macrophages in vitro.