摘要
目的 研究缺锌对生长期大鼠股骨远端骨干骨形态计量学参数的影响 ,阐明锌与骨骼发育的关系 ,为生长发育期儿童合理补充锌提供科学依据。方法 3 0只 Wistar大鼠 ,雌雄各半 ,体重 (1 0 0± 2 0 ) g,按体重随机分为三组 ,每组 1 0只 ,缺锌组和对照组饲料锌含量分别为 3 .9和2 4 .7mg/kg,对喂组喂对照组饲料 ,饲料摄入量与缺锌组相同。动物喂养 8w后处死 ,用骨形态计量学方法研究缺锌对大鼠骨组织形态学和动态学参数的影响。结果 与对照组和对喂组相比 ,缺锌组骨小梁体积显著减少、骨小梁板密度降低而间隙明显增大、骨小梁个数减少、骨形成速度降低、类骨质表面积显著减少、骨基质成熟时间明显延长、矿化沉积速率降低、矿化延迟时间增加 ,且均有显著性差异 (P<0 .0 5 )。结论 缺锌可明显降低骨形成 ,延迟骨矿化 ,破坏骨的空间结构 ,并使骨小梁连接性减弱 ,离散程度增加。
Objective:To study the effects of zinc deficiency on bone histomorphometric parameters of femoral distal diaphysis in rats. Methods:Thirty Wistar rats were randomly allocated into three groups:the zinc-deficient group(ZD), the control group(Cont),and the pair-fed group(ZP). After the rats had been fed for eight weeks, the histomorphometric and dynamic parameters of the rats were analysed using bone histomorphometric method. Results:The number, volume and connectivity of trabecular bone, and the mean trabecular plate density of ZD rats were significantly decreased, but their mean trabecular plate space was significantly increased. In addition, the data showed that ZD animals had significantly decreased trabecular osteoid surface, reduced velocity of bone formation as compared with Cont and ZP animals. The results showed that in ZD rats the mineral deposit rate was significantly slow, while the mineralization lag and osteoid maturation period were obviously prolonged. Conclusion: Zinc deficiency reduces velocity of bone formation and prolongs bone mineralization and destroys bone structure.
出处
《营养学报》
CAS
CSCD
北大核心
2002年第1期25-28,共4页
Acta Nutrimenta Sinica
基金
达能营养中心研究基金
