反相高效液相色谱法测定布比卡因血药浓度

Determination of bupivacaine in plasma by RP-HPLC

目的 :建立血浆中布比卡因反相高效液相色谱测定法 ,为临床监测血药浓度提供方法学基础。方法 :采用液 液萃取法并选用利多卡因为内标 ,以KromasilC18柱 ,流动相为甲醇 水 二乙胺 冰醋酸 (30 0∶10 0∶0 .4∶0 .2 ) ,紫外 2 30nm检测。结果 :本法在 0 .0 5～ 4 .0 μg·ml-1范围内线性良好 (r =0 .9998,n =6 ) ;最低检测限为 2 0ng(S/N≥ 3) ;绝对和相对回收率分别为(91.0± 2 .2 ) %和 (98.1± 1.0 ) % ;日内和日间精密度都小于 4 %。结论 :本方法简便可行 。

OBJECTIVE To establish an RP high performance liquid chromatographic assay for the determination of bupivacaine in plasma.METHODS The new HPLC method involved a liquid liquid extraction for plasma with lidocaine as internal standard.The sample was separated on C 18 column with a mobile phase of mixture of methanol∶water∶acetic acid glacial∶diethylamine (300∶100∶ 0.4 ∶ 0.2 ).The UV detector wavelength was set at 230 nm.RESULTS The linearity was obtained from 0.05 ～ 4.0 μg·ml -1 of bupivacaine in plasma ( r= 0.9998 , n=6 ).The determination limit was 20 ng(S/N≥3).The absolute and relative recoveries were ( 91.0 ± 2.2 )% and ( 98.1 ± 1.0 )% respectively.The RSD were less than 4%.CONCLUSIONS The method is rapid,simple and accurate.