上海部分健康体检人员中感染TTV的基因分型

Sequencing and Genotyping for TTV in Partial Healthy Population of Shanghai

目的 调查上海地区健康人群TTV感染情况及其基因分型。方法 用PCR法检测血清标本中的TTVDNA ,对TTVDNA阳性标本作核酸序列测定。结果 10 0份健康体检人员血清标本 ,用PCR法检出TTVDNA阳性标本 2 1份。其中 14份作了部分序列测定 ,10份部分序列与TTVG1a基因亚型组的TA2 78株等的同源性为 96 2 %～ 99 5 %。另 4份与G1a组同源性为 6 6 2 %～ 6 7 6 % ,与TTVG4基因型组同源性为 81 9%～ 84 8% ,与国内报道的各株TTV中同源性最大的 6 1 SEQ为 81 9%～ 84 3%。结论 上海地区部分健康人群中除分布有TTVG1a基因亚型感染外 ,还分布有国内未见报道的TTV基因型感染 ,其可归为TTVG4的一基因亚型。

Objective To investigate the TTV genotypes distribution in healthy population of shanghai.Methods TTV DNA in serum was detected by semi-nested PCR.Partial gene of TTV was sequenced directly from the PCR products,and their genetic variation was analyzed.Results There were 21 positive cases in 100 serum samples from healthy population.Fourteen positive samples were sequenced.Sequencing analysis showed that 96.2%-99.5% of nucleotides were analogous between 10 cases and TA278,CMR12,66.2%-67.6% between another 4 cases and the two strains,but 81.9%-84.8% in the 4 cases with TTV G4 strains.The most analogous to the 4 cases in TTV strains reported in China was 61.SEQ,and the analogous rate was 81.9%-84.3%.Conclusion The prevalance of TTV was high in Shanghai healthy population.TTV G1a was detected as main subgenotype and a subgenotype of TTV G4 new reported in China distribut in Shanghai healthy population.