高温与低剂量香烟联合致仔鼠神经行为异常研究

Study on Nurobehavioral Abnormal of Offspring of Rats Exposed to Hyperthermia and Cigarette Smoking

目的 探讨高温与香烟联合对仔鼠神经行为发育的影响。方法 孕 10天的大鼠暴露于高温 (41℃和 4 2℃ ) ,同时在孕 2 ,4 ,6 ,8,10 ,12 ,14天时注射低剂量的香烟烟雾水溶物 (1 0支 /只 ) ,观察分析仔鼠体格发育、反射行为及学习记忆等神经行为。结果 高温组 (41℃和 4 2℃ )仔鼠体格发育 (开眼、张耳、生毛、出牙 )和反射感觉功能 (平面翻正、空中翻正、视觉定向和听觉惊愕 )的达标天数及学习与记忆能力达标时间 (分 )与对照组及 37℃组比较明显延迟 (P <0 0 1) ;同时还表明持续较长时间的高温 (>1 5分钟 )与香烟联合对仔鼠上述神经行为具有复合作用 ,与单独高温组比较有显著性差异 (P <0 0 1)。结论 持续较长时间的高温与香烟联合可使仔鼠生长发育延缓及学习记忆功能降低等神经行为异常。

Objective To study the effect of the hyperthmia and cigarette smoking on the neonate rat′s neurobehavioral.Methods Rats pregnant for 10 days were exposed to high temperature(41℃ or 42℃).Water soluble substances containing cigarette smoking(1 0 cigarette/20 g rat) were given through subcutaneous inject at 2,4,6,8,10,12,14 days of gestation.The neonate rat′s neurobehavioral (the appearance of four physiological markers,the acquisition of four reflexes,learning and memory)was observed.Results In contrast to the control group and 37℃ group,when pregnant rats were exposed to high temperature(41℃ and 42℃) the neonate rats were delayed for the appearance of four physiologic markers(eye opening?pinna detachment?hair growth and tooth growth),the age of acquisition of four reflexes(surface righting,air righting,virual placing and auditory orienting)and the time of learning and memory( P <0 01).There was significant combined effect between the sustained hyperthermia of longer time and cigarette smoking,and there was a significant difference compared with the neurobehavioral of those only in single hyperthermia( P <0 01).Conclusion The sustained hyperthermia combined with cigarette smoking can increasely delayed the neurobehavior growth of neonate rats.