McAb-ELISA检测丝虫特异IgG_4的应用研究

Study on Application of McAb-ELISA to Filaria Surveillance by Detecting Filaria-specSifie IgG_4

目的 探讨McAb -ELISA检测丝虫特异IgG4在丝虫感染监测中的应用价值。方法 利用生化免疫技术 ,经细胞融合 ,建立起 7株对IgG4表现为单一反应性的单克隆抗体 (McAb)。以此为探针 ,采用ELISA方法检测丝虫特异IgG4。结果 检测班氏微丝蚴血症者血清的敏感性为 96 3% (10 4 / 10 8) ,测得IgG4的最低限量为 0 0 1ul/ml;检测丝虫病非流行区健康者、肠道线虫感染者、华支睾吸虫感染者和囊虫病患者等血清均呈阴性 ,未出现交叉反应 ,特异性为 10 0 %。经现场应用研究 ,同样获得良好的实用性 ,有效地反映出当地丝虫病流行与感染状况。结论 该技术是监测丝虫感染的理想方法。

Objective To discuss the practical value of McAb-ELISA to detect filaria speicfic IgG 4.Methods Seven strains of filaria-specific McAb were established through technique of cellular fusion and biochemistry-immunology, and were used in filarial infection test as probes to detect filaria-specific IgG 4.Results Its sensitivity was 96 3%(104/108).It detected as low as 0.01μl/ml IgG 4 per milliliter serum.No cross-action were tested with serums collected from residents of non-endemic area,including those infected with intestinal nematodes,liver fluke and systicercus.Its specificity was 100%.It was also feasible and practical when applied to field study,effectively reflected the epidemic situation of local filarial infection.Conclusion This was an ideal method to be used in filarial infection surveillance.