摘要
目的 探讨McAb -ELISA检测丝虫特异IgG4在丝虫感染监测中的应用价值。方法 利用生化免疫技术 ,经细胞融合 ,建立起 7株对IgG4表现为单一反应性的单克隆抗体 (McAb)。以此为探针 ,采用ELISA方法检测丝虫特异IgG4。结果 检测班氏微丝蚴血症者血清的敏感性为 96 3% (10 4 / 10 8) ,测得IgG4的最低限量为 0 0 1ul/ml;检测丝虫病非流行区健康者、肠道线虫感染者、华支睾吸虫感染者和囊虫病患者等血清均呈阴性 ,未出现交叉反应 ,特异性为 10 0 %。经现场应用研究 ,同样获得良好的实用性 ,有效地反映出当地丝虫病流行与感染状况。结论 该技术是监测丝虫感染的理想方法。
Objective To discuss the practical value of McAb-ELISA to detect filaria speicfic IgG 4.Methods Seven strains of filaria-specific McAb were established through technique of cellular fusion and biochemistry-immunology, and were used in filarial infection test as probes to detect filaria-specific IgG 4.Results Its sensitivity was 96 3%(104/108).It detected as low as 0.01μl/ml IgG 4 per milliliter serum.No cross-action were tested with serums collected from residents of non-endemic area,including those infected with intestinal nematodes,liver fluke and systicercus.Its specificity was 100%.It was also feasible and practical when applied to field study,effectively reflected the epidemic situation of local filarial infection.Conclusion This was an ideal method to be used in filarial infection surveillance.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2002年第5期579-580,共2页
Chinese Journal of Public Health