蛋白胨对臭氧水杀菌作用影响的试验研究

Study on Influences of Peptone on Germicidal Efficiency of Ozone Solution

目的 采用北京国源消毒技术研究所提供的高浓度臭氧水生成机,通过实验室试验,观察了蛋白胨对臭氧水杀菌作用的影响,为消毒实际应用提供实验室试验依据。方法 测定臭氧水中加入1％蛋白胨后残留臭氧浓度的变化;以及菌悬液中加入1％蛋白胨后对臭氧水杀菌作用的影响。按照《消毒技术规范（第3版）第一分册试验技术规范》（1999.12）的要求配制含蛋白胨的菌悬液。用碘量法测定臭氧水浓度,杀菌实验采用悬液定量法。结果 研究表明,臭氧水中加入1％蛋白胨后,残留臭氧浓度迅速降低;菌悬液中加入1％蛋白胨后,臭氧水对大肠杆菌的杀灭率显著下降;消毒试验所用臭氧水体积增加到50ml时,作用1min,对大肠杆菌的杀灭率可达99.93％。结论 臭氧水的杀菌作用受蛋白胨影响较大,消毒实际应用时,为保证消毒效果,可通过增加臭氧水的用量来克服有机物的影响。

ve To provide some laboratory data for using ozone solution as a disinfectant, the influences of peptone on germicidal efficiency of ozone solution were observed using generator provided by Guoyuan Technological Institute on Disinfection of Beijing. Methods According to the procession and requirement in 'Technological Specification on Disinfection (the third version) :The first handbook: Technological Specification on Experiment' (1999.12), germicidal experiment were taken with method of fix - quantity in solution, concentration of ozone was measured with iodine - quantity method. Results The residual ozone in ozone solution and the germicidal effieiency of ozone solution could be strongly lowred by presence of 1 % peptone. Increase in the volume of the ozone solution to 50ml, the killing rates could reach to 99. 93 % after 1min. Conclusion Considering the strong influence of organic substance on ozone solution, the volume of the ozone solution should be increased when using ozone solution as a disinfectant in order to assure the germicidal effieiency in actual use.