5种蟋蟀的RAPD研究初报(直翅目:蟋蟀总科)

Random amplified polymorphic DNA in five species of crickets (Orthoptera:Grylloidea)

应用RAPD技术对 5种蟋蟀的基因组DNA进行扩增 ,从 1 0种随机引物中筛选出一种引物S8可以对 5个种扩增出稳定且相异的DNA图谱 ,每个种的DNA扩增条带的相对分子质量为 :长颚斗蟋V .aspersusS8- 1 90 0 ,1 51 0 ,1 3 2 0 ,83 0 ,740 ,3 70 ;小斗蟋V .parvusS8- 1 90 0 ,1 1 50 ,83 0 ,60 0 ,3 50 ;石首棺头蟋L .equestrisS8- 1 90 0 ,960 ,560 ;多伊棺头蟋L .doenitziS8- 1 90 0 ,80 0 ;黄脸油葫芦T .emmaS8- 1 490 ,1 2 90 ,1 1 80 .结果表明 ,引物S8可用于

The RAPD protocols is first used to study the genomic DNA of 5 species of crickets. The genomic DNA of 5 species of crickets is amplified with 10 different oligo nucleotide 10 mer primer. Primer S8 is screened by RAPD PCR and five separate DNA maps are obtained through amplifying the genomic DNA of the crickets, lengths of the DNA bands of each map: Velarifictorus. asperses S8-1 900, 1 510 , 1 320, 830, 740, 370, V.parvus S8-1 900, 1 150 , 830, 600, 350, L.equestris S8-1 900, 960, 560, L.doenitzi S8-1 900, 800, T.emma S8-1 490, 1 290, 1 180. The study also shows that amplified DNA exist sexual and individuals differences, but these differences distinctly differ from those among the species. The test results are stable and reproducible. Prime S8 could be used for the identification of five species of crickets.