新生儿缺氧缺血性脑病外周血T细胞亚群IL-6 IL-8水平变化及临床意义探讨

The clinical significance of T lymphocytic subgroups and interleukin-6,interleukin-8 levels in peripheral blood of neonatal hypoxic ischemic encephalopathy

目的 观察新生儿缺氧缺血性脑病 (HIE)外周血T细胞亚群、白细胞介素 (IL) 6、IL 8水平变化 ,并探讨其临床意义。方法 收集 5 0例HIE患儿和 30名正常新生儿外周血 ,采用双色免疫荧光标记法及流式细胞仪检测CD3+ 、CD4 + 、CD8+ T细胞免疫表型 ,采用酶标免疫吸附法检测IL 6、IL 8。记录HIE患儿临床资料。结果 HIE组外周血CD3+ 、CD4 + 、CD8+ 细胞水平低于对照组 ,IL 6、IL 8高于对照组 ,差异有高度显著性 (P <0 0 1) ;不同病情、不同转归HIE组CD3+ 、CD4 + 、CD8+ 、IL 6差异有高度显著性 (P <0 0 1) ,即CD3+ 、CD4 + 、CD8+ 降低 ,IL 6升高越显著者 ,病情越重 ,预后越差。结论 新生儿HIE免疫功能受损 ,CD3+ 、CD4 + 、CD8+ 细胞和IL 6、IL 8水平对临床早期判断病情严重性、估计预后、指导治疗有一定意义。

Objective To study the changes of T cell subgroups and interleukin (IL) 6,IL 8 levels in peripheral blood of neonates with hypoxic ischemic encephalopathy (HIE),the relationship between brain injuries of HIE and the parameters,and their clinical significance Methods Peripheral blood samples of 50 HIE cases and 30 normal neonates were collected.Immunophenotypes of T cell were determined by double immunofluorescence labeled assay with flow cytometry The levels of IL 6 and IL 8 were determined by enzyme linked immunosorbent assay (ELISA) Clinical data of HIE neonates were collected and analysed Results Peripheral blood CD3 + ,CD4 + ,CD8 + T cell levels in HIE group were significantly lower than those in normal control,while IL 6,IL 8 levels in HIE group were significantly higher than those in normal control The levels of CD3 + ,CD4 + ,CD8 + T cell and IL 6 were significantly related to disease severity and different prognostic cases,i e ,patient who had lower CD3 + ,CD4 + ,CD8 + levels but a higher IL 6 level might be more serious and of worse prognosis Conclusion Immune function in neonatal HIE was impaired The levels of CD3 + ,CD4 + ,CD8 + T cell and IL 6,IL 8 could be markers for assessing the disease severity and predicting outcome