自制声学造影剂定量局部心肌血流效果的实验研究

Assessment of regional myocardial blood flow by self-made microbubble contrast agent

目的 探讨自制声学造影剂进行心肌造影超声心动图 (myocardialechocardiography ,MCE)检查中自制声学造影剂定量局部心肌血流 (myocardialbloodflow ,MBF)的能力。 方法 MCE检测开胸犬不同状态的局部MBF ,并与放射性微球测得的心肌血流量的比较。①造影剂制备 :将 1%人体白蛋白、5 %葡萄糖和氟碳气体以一定的比例混合 ,分别以 70s ,14 0s和 2 10s进行声振 ,比较各组造影剂中微泡的大小和浓度。②MCE定量MBF :开胸犬 6条 ,分别在基础状态、静脉给予潘生丁造成的充血状态、左冠状动脉回旋支 (LCx)不同程度狭窄状态下计算MCE得出的局部MBF。③在最后狭窄状态 ,于MCE结束后左房内注入放射性微球测量局部心肌血流量。结果 ①以 70s ,14 0s和 2 10s声振制成造影剂微泡的直径分别为 ( 9.0 1± 3.14 ) μm ,( 6 .45± 2 .39) μm ,( 4 .86± 2 .0 9) μm ,浓度分别为 ( 1.71± 0 .2 2 )× 10 8个 /ml,( 3.77± 0 .92 )× 10 8个 /ml,( 8.95± 0 .81)× 10 8个 /ml ,故使用与文献中造影剂直径和浓度最接近的 2 10s声振造影剂进行MCE。②在无冠脉狭窄及狭窄程度较轻时 ,随触发间歇时间的延长 ,心肌最后均可达完全显影 ,但所需时间不同。③A·β(LCx) /A·β(LAD) 与放射微球测得MBF(LCx) /MBF(LAD) 相关 (Y

Objective Using self-made microbubble contrast agent to quantify the change of the regional myocardial blood flow (MBF) with myocardial contrast echocardiography (MCE). Methods ①Sonicated the mixture of 1% albumin, 5% dextrose and perfluorocarbon gases using 70, 140 and 210 seconds, and compared the size and concentration of the three kind of microbubbles. ②MCE was performed in 6 open chest dogs during baseline, hyperemia by dipyridamole and different grades of coronary stenosis and regional MBF was calculated. Results ①The microbubble size of the 3 groups was ( 9.01 ± 3.14 )μm, ( 6.45 ± 2.39 )μm, ( 4.86 ± 2.09 )μm, respectively, and the concentration was ( 1.71 ± 0.22 )×10 8 microbubbles/ml, ( 3.77 ± 0.92 )×10 8 microbubbles/ml, ( 8.95 ± 0.81 )×10 8 microbubbles/ml, respectively. And group 3 was used. ②Myocardial bed could be completely fulfilled by the contrast agent when coronary stenosis was none or mild, although the time needed was different. ③MBF derived from MCE had good correlations with those derived from radiolabeled microspheres ( Y = 0.76 X + 0.07 , r = 0.82 , P = 0.01 ). Conclusions MCE with self-made microbubble contrast agent can quantifiably reflect regional MBF.