吸入一氧化氮对吸入性损伤大鼠肝脏毒副作用的研究

The Toxicity and Side Effect of Nititric Oxide to Rat's Liver with Inhalation Injury

目的:观察吸入外源性一氧化碳(NO)对重度烟雾吸入性损伤大鼠肝脏的毒副作用。方法:72只大鼠随机分为3组,均行气管切开及插管,制成重度吸入性损伤,予以呼吸机辅助呼吸。对照组(G_1)单纯吸氧(FiO_20.4),低浓度NO吸入组(G_2)和高浓度NO吸入组(G_3)除吸氧外,分别吸入40×10E^(-6)和80×10E^(-6)的NO,于伤后2、8、12、24h取标本检测。另取6只正常大鼠和6只致伤后半小时大鼠分别建立正常值与伤后值。数据行多个样本均数间方差分析。结果:G_1组GTP明显高于G_2和G_3组及伤前值(P<0.01),G_1和G_3组Tbil均高于G_2组和伤前值(P<0.05)。G_2组织含水量低于G_1和G_3组(P<0.01)。G_1组MHb高于G_2和G_3组(P<0.01),G_3组呼出气中NO_2浓度高于G_1和G_2组(P<0.01)。G_2的组织形态和超微结构的病理改变明显轻于G_1,G_3组次之。结论:吸入40×10E^(-6)的NO能减轻吸入伤后肝脏组织的病理改变,而80×10E^(-6)的NO则可能有一定的损伤作用。

Objective: To evaluate the toxicity and side effect of exogenous nitric oxide (NO) to the rat's liver with smoke inhalation injurey.Method:72 rats were divided into three groups,a tracheostomy was performed and a trachea! tube was placed for each animal in three groups.Group1(G1, n = 24) .breathed in O2(FiO2 = 0.40) ;group2(G2, n = 24)breathed in O2(FiO2 = 0.40) + 40 × 10E-6NO; group3(G3,n = 24) breathed in O2(FiO2 = 0.40) + 80 × 10E-6 NO. In addition, 6 animals without inhalation injury served as normal controls, and another 6 animals subjected to inhalation injury were sacrificed at 30 minutes. The data was analyzed using ANOVA for multiple mean comparison. Results: GTP of G1 was higher markedly than G2 、 G3 and normal value ( P < 0.01) , TbiL of G1 and G3 was higher than G2 and normal value ( P < 0.05). The quantity of water in the cardiac muscle tissue of G2 was lower than G1 and G3 ( P < 0.01). MHb of G1 was higher than G2 and G3 ( P < 0.01) . The concentration of NO2 of G3, was significantly higher than that of G1 and G2 ( P < 0.05 and P < 0.01) . The result of comparison of morphology and ultrastructure of liver of G2 was better than that of G1, and second the G). Conclusion: Inhaling 40 × 10E-6 NO can reduce the pathology changes of live injured with smoke inhalation, but inhaling 80 × 10E-6 NO may exert some slight side harmful effect.