小鼠腹腔注射阿霉素的急毒实验及其对外周血的影响

Adriamycin's acute toxicity in mice through celiac injection and its effect on the peripheral blood

目的 测定雄性小鼠 ip阿霉素 (ADM)急毒 L D50 及对外周血影响的剂量效应关系 .方法 昆明种小鼠随机分成8组 ,阴性对照组和 ADM7个不同剂量组 ,除阴性对照组外 ,其余依次经 ip1.2 5 ,2 .5 0 ,5 .0 0 ,10 .0 0 ,15 .0 0 ,2 0 .0 0和 40 .0 0mg· kg- 1 7个不同剂量的 ADM,阴性对照组给予对应剂量的生理盐水 ,观察动物给药后 10 d内的生存情况 .结果 ADM的 7个剂量组动物的死亡率随 ADM的剂量由低到高依次是 0 ,0 ,0 ,2 0 % ,70 % ,90 %和 10 0 % .ADM可引起外周血除红细胞分布活力 (RDW)、平均血小板体积 (MPV )和淋巴细胞百分比 (L YM)外几乎所有的指标降低 ,红细胞 (RBC)、白细胞 (WBC)、血小板 (PL T) 3种血液成分降低显著 (P<0 .0 5 ) ,并随 ADM剂量的增加而愈发明显 .结论 雄性昆明种小鼠 ADM ip L D50 为 13.2 mg· kg- 1 ,其 95 %可信限范围为 10 .6～ 16 .4mg· kg- 1 . ADM毒性导致的血液

AIM To investigate adriamycin's acute toxicity in mice through ip and its effect on the peripheral blood. METHODS The animals were randomly divided into eightgroups which were negative control group and seven adriamycin (ADM) groups. The seven ADM groups were given ADM through ip at the dosage of 1.25, 2.50, 5.00, 10.00, 15.00, 20.00 and 40.00 mg·kg -1 respectively, then we observed the survival status in the following ten days. RESULTS The animals death rates in different ADM groups were 0, 0, 0, 20%, 70%, 90% and 100% sequentially from the low dosage to high. Except RDW, MPV and LYM parameters, ADM could induce almost every parameter, especially the RBC, WBC and PLT, to decrease significantly, which was more clear with the increasing of ADM dosage. CONCLUSION LD 50 of ADM ip in male Kunming mice is 13.2 mg·kg -1 , with 95% confidence interval of 10.6～16.4 mg·kg -1 . ADM induces all kinds of blood cells to decrease significantly.