绞股蓝总苷防治大鼠肝纤维化的实验研究

Protective Effect of Gypenosides on Experimental Hepatic Fibrosis in Rats

目的 :观察绞股蓝总苷对肝纤维化的防治作用并探讨其机理。方法 :用 5 0 %四氯化碳油腹腔注射6周诱发大鼠肝纤维化模型 ,同时用绞股蓝总苷 (10 0～ 2 0 0 mg/ kg,ip)治疗。测定肝功能 ,肝组织内丙二醛 (MDA)和超氧化物歧化酶 (SOD)水平 ,并通过光镜、电镜观察肝组织病理变化 ,免疫组织化学法检测肝组织纤维连接素 (Fn)表达。结果 :绞股蓝组较模型组 :肝功能明显改善 ,谷丙转氨酶 (AL T)、谷草转氨酸 (AST)显著降低 ;肝组织纤维化程度明显改善 ;肝组织内 MDA水平明显降低 ,SOD水平明显升高 ;肝组织内 Fn表达减少。结论 :绞股蓝总苷具有抗肝纤维化作用 ,此作用可能与其保护肝细胞 。

Objective:To study the effect of gypenosides (GP) on experimental hepatic fibrosis in rats.Methods:The rat hepatic fibrotic model was induced by the peritoneum injection of 50% CCl 4 (twice a week for 6 weeks). The fibrotic rats were treated with low-dose and high-dose GP (100～200 mg/kg ip, once a day for 42 days) during inducing animal model. Liver function and level of MDA and SOD in liver tissue were determined. Histopathological changes were examined by optical microscopy and electronic microscopy. The expression of fibernectine (Fn) in liver tissue were detected by immunohistochemical techniques.Results:Compared with model group, it revealed that in GP-treated rats:Liver function was improved, alanine transaminase (ALT) and aspartate aminotransferase (AST) obviously decreased (P< 0.05 or P<0.01). Level of MDA in liver tissue was markedly reduced (P<0.05 or P<0.01). The activities of SOD in liver tissue was significantly elevated. The degrees of fibrosis were reduced (P<0.05 or P<0.01). The expression of Fn in liver tissue were ameliorated.Conclusion:GP has an protective effect on hepatic fibrosis in rats. The effect may be related to its preventing liver cell against injury and inhibiting liver Fn expressions.