铅对染毒大鼠血清超氧化物歧化酶活性和丙二醛含量的变化

The changes of serum SOD activity and MDA content in rats induced by lead

目的 研究慢性染铅大鼠血清超氧化物歧化酶 (SOD)、丙二醛 (MDA)的变化及其与血铅的剂量 -效应关系 ,以探讨铅中毒的作用机制。方法 SOD活性测定采用亚硝酸盐显色法 ,MDA含量测定采用硫代巴比妥 (TBA)法。结果 与对照组相比 ,低剂量染铅组大鼠血清SOD活性在 60d以后明显下降 (P <0 .0 5 ) ;高剂量染铅组SOD活性 3 0d以后明显降低 (P <0 .0 5 ) ,MDA亦明显高于对照组 (P <0 .0 5 )。血清SOD与血铅之间存在负相关 (r =-0 .479,P <0 .0 1)且呈现负向剂量 -效应关系 ;血清MDA与血铅之间存在正相关关系 (r =0 .496,P <0 .0 1)且呈现正向剂量 -效应关系。结论 慢性染铅可导致血清SOD活性降低、MDA含量升高 ,且呈剂量 -效应关系。

Objective To study the change of superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in serum of rats exposed to lead, and the dose effect relationship between lead and SOD, MDA respectively. Methods SOD activity in serum was measured by nitrite coloration and MDA contents were determined by TBA method. Results Compared with control groups, the activities of serum SOD were markedly decreased in low dose groups after 60 days lead exposure, and 30 days in high dose groups( P < 0.05 ). Serum MDA contents in high dose groups were higher than those of control groups after 30 days. There were negative correlation( r =-0.479, P <0.01) and negative dose effect relationship between SOD in serum and lead in blood; there were positive correlation( r=0.496,P<0.01) and positive dose effect relationship between MDA and lead. Conclusion SOD activity appeared to be significantly decreased and MDA content significantly increased during sub chronic lead exposure and there was dose effect relationship between them. It suggested that lipid peroxidation could be one of molecular mechanism in lead poisoning.