核因子-κB与大肠肿瘤细胞凋亡及增生的关系

Relationship between nuclear factorkappa B, apoptosis and proliferation in colorectal neoplasia

目的:通过观察大肠腺瘤、腺癌和正常大肠组织中的细胞凋亡、细胞增生及其调控基因Bcl-2,Bcl-Xl,C-myc和核因子-κB的表达状态,探讨核因子-κB在大肠癌发病过程中的作用。 方法:利用DNA缺口末端标记技术和NF-κB,Bcl-2,Bcl-Xl,C-myc,PCNA蛋白免疫组化染色,原位检测30例大肠腺瘤和30例大肠腺癌中凋亡细胞、细胞增生和NF-κB,Bcl-2,Bcl-Xl,C-myc阳性表达细胞的密度和分布,以10例正常大肠组织作对照。 结果:NF-κB,Bcl-2,Bcl-Xl,C-myc蛋白的表达率和表达强度腺癌和腺瘤高于正常组织(70.9±7.2 vs 54.0±4.5 vs 30.6±2.4;78.2±8.3 vs 55.6±6.5 vs 11.0±1.9;77.3±6.5 vs 56.4±4.7vs 9.3±1.1;70.3±6.7 vs 50.1±4.2 vs 14.2±1.7,P<0.01),腺癌高于腺瘤(70.9±7.2 vs 54.0±4.5;78.2±8.3 vs 55.6±6.5;77.3±6.5 vs 56.4±4.7;70.3±6.7 vs 50.1±4.2,P<0.01)。腺瘤和腺癌中的凋亡细胞指数均高于正常组织(105.9±6.1 vs31.5±3.7 vs 10.0±1.5,P<0.01),腺瘤高于腺癌(105.9±6.1vs 31.5±3.7,P<0.01)。腺癌和腺瘤中的细胞增生高于正常组织(75.4±5.6 vs 50.7±4.9 vs 29.6±3.0,P<0.01);腺癌高于腺瘤(75.4±5.6 vs 50.7±4.9,P<0.01)。 结论:NF-κB在大肠肿瘤中促进细胞增生、抑制细胞凋亡。

AIM:To investigate the effects of nuclear factor-kappa B in the stage of malignant transformation of large intestinal epithelium.METHODS:The density and distribution of the proliferation, apoptosis and the positive expression of NF-KB,Bcl-2,Bcl-XI and C-myc protein were observed in situ in 30 cases of colorectal adenomas and 30 colorectal adenocarcinomas. 10 cases of normal mucosa were used as controls.RESULTS: The positive rate and staining intensity of NF-KB, Bcl-2, Bcl-XI and C-myc protein in adenocarcinoma and adenoma were higher than in normal mucosa(70.9 ± 7.2 vs 54.0±4.5 vs30.6±2.4;78.2±8.3 vs55.6±6.5 vs11.0±1. 9;77.3±6.5 vs 56.4±4.7 vs 9.3 ± 1.1;70.3 ± 6.7 vs50.1± 4.2 vs 14.2 ± 1.7, P < 0.01). Their staining intensity in adenocarcinoma was higher than that in adenoma(70.9 ±7.2vs 54.0±4.5;78.2±8.3 vs 55.6 ± 6.5;77.3 ± 6.5 vs 56.4± 4.7;70.3 ± 6.7 vs 50.1± 4. 2, P < 0. 01). The density of apoptotic cells in adenoma and adenocarcinoma were higher than that in normal mucosa(105.9±6.1 vs 31.5±3.7 vs 10. 0±1.5,P<0.01), and their density in adenoma were higher than that in adenocarcinoma(105.9±6.1 vs 31.5±3.7, P< 0.01). The degree of proliferation in adenocarcinoma and adenoma were higher than that in normal mucosa(75.4± 5.6vs 50.7 ±4.9 vs 29.6 ± 3.0, P< 0.01), in which that in adenocarcinoma were higher than in adenoma(75.4± 5.6 vs 50.7±4.9,P<0.01).CONCLUSIONS: NF-KB can enhance proliferation and inhibit apoptosis, which may play an important role in the pathogenesis of colorectal carcinoma.