摘要
为探讨鼻腔接种伤寒杆菌Fe SOD对鼠伤寒杆菌攻击小鼠的交叉免疫保护作用 ,用IL 1作为佐剂 ,将伤寒杆菌Fe SOD经鼻腔接种小鼠 ,再以不同剂量的鼠伤寒杆菌攻击 ,观察小鼠的存活情况。当用IL 1作为佐剂时 ,经鼻腔接种伤寒杆菌Fe SOD的小鼠在 2LD50 鼠伤寒杆菌攻击后 ,3天和 7天的存活率均明显高于对照组 (P <0 0 1或P <0 0 5 ) ,当以 5LD50 鼠伤寒杆菌攻击时 ,小鼠 7天的存活率明显高于对照组 (P <0 0 1)。结果说明伤寒杆菌Fe SOD经鼻腔接种后对鼠伤寒杆菌的攻击可产生一定的免疫保护作用 ,也进一步说明了Fe SOD是沙门氏菌的共同保护性抗原。
In this study,the active cross immuno protection of intranasal administration was carried out by S.typhi Fe SOD for mice against challenge with S.typhimurium.The mice were immunized with S.typhi Fe SOD intranasally with IL 1 as a adjuvant,then the mice were challenged with different doses of S.typhimurium,and the survival rates of mice were recorded.The survival rates of mice in the group immunized with S.typhi Fe SOD with IL 1 challenged with 2 LD 50 S.typhimurium were higher than that of control group during a period of 3 days and 7 days respectively,and the survival rates of mice in the experiment group challenged with 5LD 50 S.typhimurium were also higher than that of control group during a period of 7 days.Intranasal immunization with S.typhi Fe SOD could confer active cross immuno protection to mice challenged with S.typhimueium.The results showed that SOD was the common protective antigen of Salmonella.
出处
《微生物学免疫学进展》
2002年第2期11-13,共3页
Progress In Microbiology and Immunology
基金
广东医学院青年科学基金资助项目编号 :青 990 8
