Leptin及其受体的表达与肝癌血管生成、生长和转移的关系

Relationships of the Expression of Leptin and its Receptor between Angiogenesis, Growth and Metastasis in Human Hepatocellular Carcinoma

目的 探讨 L eptin及其受体的表达与肝癌血管生成和肿瘤生长、转移的关系。 方法 应用免疫组织化学方法观察 95例原发性肝癌、6 0例肝硬化、40例正常肝组织 L eptin及其受体 (Ob- R)的表达情况 ,分析 L eptin、Ob- R与微血管密度 (MVD)及肿瘤细胞增殖指数的关系。 结果 肝癌细胞上 L eptin呈强阳性表达者 ,其 MVD明显高于弱阳性表达及阴性者 (P<0 .0 5 ) ,两者呈正相关关系 (r=0 .42 78,P<0 .0 5 ) ;肝癌血管内皮细胞中 L eptin阳性表达者的 MVD明显高于阴性者 (P<0 .0 1) ,呈正相关关系 (r=0 .42 19,P<0 .0 5 ) ,且与肿瘤转移倾向成正相关 (P<0 .0 1) ;肝癌细胞中 Ob- R表达与转移的发生呈正相关关系 (P<0 .0 1) ,在肝癌组织中也可见 Ob- R在血管内皮细胞中的表达。 结论 L eptin在肝癌的血管生成及转移过程中可能起着重要的作用。

Objective To investigate the relationships among the expression of leptin, angiogenesis, growth and metastasis in human hepatocellular carcinoma(HCC). Methods 95 HCC cases, 60 liver cirrhosis cases and 40 normal liver tissue samples were stained with antibodies against leptin or its receptor(Ob R) by immunohistochemistry, and the correlation with microvessel density(MVD) and proliferative index were analyzed. Results In HCC, positive correlation between the expression of leptin in tumor cells and MVD was observed(r=0 4278,P<0 05). The expression of leptin was observed in endothelial cells, and MVD and metastatic frequency were greater in positive expression of leptin than in negative expression(P<0 01 respectively). The expression of Ob R in tumor cells was positively correlated with metastatic frequency of HCC(P<0 01), and endothelial cells showed a little Ob R expression. Conclusion leptin may play important roles in angiogenesis and metastasis in HCC.