摘要
AIM A RP HPLC method was developed for the determination of nicotinamide and vitamin B 6 in compound vitamin B injection. METHODS The sample was analysed on C 18 column. The mobile phase is acetonitrile 0.01 mol/L Na 2SO 4 solution (5∶95) adjusted at pH 3.4 by sulfate acid and the detective wavelength is 254 nm. RESULTS A good linearity can be found in the range of 4.26～24.36 μg/ml and 40.26～240.1 μg/ml. The recovery of nicotinamide and vitamin B 6 was 97.53%, 97.87% respectively and the RSD (%) was not more than 1.0. CONCLUSION The method was found to be simple, rapid and reproducible for determination of nicotinamide and vitamin B 6 in compound vitamin B injection.
AIM A RP HPLC method was developed for the determination of nicotinamide and vitamin B 6 in compound vitamin B injection. METHODS The sample was analysed on C 18 column. The mobile phase is acetonitrile 0.01 mol/L Na 2SO 4 solution (5∶95) adjusted at pH 3.4 by sulfate acid and the detective wavelength is 254 nm. RESULTS A good linearity can be found in the range of 4.26～24.36 μg/ml and 40.26～240.1 μg/ml. The recovery of nicotinamide and vitamin B 6 was 97.53%, 97.87% respectively and the RSD (%) was not more than 1.0. CONCLUSION The method was found to be simple, rapid and reproducible for determination of nicotinamide and vitamin B 6 in compound vitamin B injection.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2002年第2期161-163,共3页
Journal of China Pharmaceutical University
