脑血管痉挛后血管平滑肌细胞中c-fos基因表达及意义

Expression of c-fos gene and its role in vascular smooth-muscle cells after cerebral vasospasm

目的 探索脑血管平滑肌细胞c-fos早期快反应基因与脑血管痉挛的关系。方法 新西兰兔36只,随机等分为3组:实验组、新霉素组和对照组。实验组和新霉素组采用二次注血模型,新霉素的静脉注射给药剂量根据预实验确定为3mg·kg-1,每天2次。分别于12h、2d和7d处死动物,脑标本经灌注固定后行c-Fos免疫组化染色。结果 实验组c-fos早期快反应基因的表达强度（75％）明显高于新霉素组（25％）和对照组（17％）（P=0.032）,c-Fos表达在蛛网膜下腔出血后2d内最显著,并持续7d以上,而血管性病理损害发生在2d以后（P=0.005）。结论 c-fos早期快反应基因与蛛网膜下腔出血后脑血管痉挛病理改变有关。

Objective To explore the relationship between the c-fos immediate early gene expression in vascular smooth-muscle cells and cerebral vasospasm. Methods Thirty-six New Zealand rabbits were randomly divided into the experimental group, the neomycin group and the control group. Experimental group model was produced with modified two-hemorrhage method. The neomycin of 3mg·kg-1 was injected intravenously, twice a day, which was based on pre-experiment. The animals were sacrificed 12h, 2d, 7d after SAH, and then the brains were taken out for pathological examinations. The c-Fos protein was examined by immunohistochemistry in vascular smooth-muscle cells. Results The c-fos gene expressive rate was higher in the experimental group (75%) than in the neomycin (25%) and control (17%) group (P =0.032). The c-Fos expression increased sharply within 2 days after SAH and dropped 7days after SAH. Morphological changes in cerebral arteries were observed at 2 days after SAH ( P = 0. 005). Conclusion The c-fos gene expression is responsible for the pathological changes in the cerebral vasospasm after subarachnoid hemorrhage.