摘要
目的:构建TCR Vβ8 ＋IL－2/pcDNA3.1亚克隆重组质粒。方法:先将TCR Vβ8克隆人克隆载体pUC19,分别酶切 IL－2/pUC19和 TCR Vβ8/pUC19,将 IL－2连接到TCR Vβ8/pUC19重组质粒上,然后将 TCRVβ8＋ IL－2基因从 TCR Vβ8+IL－2/pUC19重组质粒上酶切下,克隆入pcDNA3．1表达载体上。结果和结论:经测序证实成功地构建了 TCR Vβ8＋IL－2/pcDNA3．l亚克隆重组质粒。
Aim: To construct the subclone recornbinant plasmid of TCR Vβ8 IL-2/pcDNA3. 1. Methods:Gene of TCR Vβ8 was cloned into the cloning vector pUC19 ;IL-2/pUC-19 and TCR Vβ8/pUC-19 were cleaved with endonu-clease, respectively; gene of IL-2 was linked to the recornbinant plasmid of TCR Vβ8/pUC-19. Then gene of TCR Vβ8 + IL-2 was cleaved with endonuclease from the recornbinant plasmid of TCR Vβ8 + IL-2/pUC-19 and cloned into expressing vector pcDNA3. 1. Results and Conclusion: The subclone recornbinant plasmids of TCR Vβ8 + IL-2/pcD-NA3. 1 had been constructed and identified by sequencing.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2002年第3期277-280,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
博士基金资助课题
