高压氧对牙周炎组织前列腺素的作用及机理分析

Effects and mechanism of hyperbaric oxygen on prostaglandins in alveolar bone and gingival of experimental periodontitis in animal

目的 探讨高压氧 (hyperbaricoxygen ,HBO)对鼠牙龈和牙槽骨中前列腺素E2(prostaglandinsE2 ,PGE2 )的作用 ,研究HBO治疗牙周炎的机理。方法 对 6 0只豚鼠进行丝线缝扎和高糖食料喂养形成牙周炎 ,每天用 0 2 5MPaHBO治疗牙周炎 6 0min ,连续治疗 2周 ,用酶联免疫测定法分析牙龈和牙槽骨中PGE2 的含量 ,观察治疗组与对照组的PGE2 变化。结果 对照组鼠牙龈组织中的PGE2 含量为 3 2 1ng/g ,牙槽骨中为 3 2 2ng/g ;牙周炎形成后牙龈组织和牙槽骨中的PGE2 含量均明显升高 (P <0 0 1) ;经HBO治疗后牙龈和牙槽骨中的PGE2 含量较牙周炎组分别降低了 6 2 7%和 6 9 6 % ,且与牙周炎组相比差异有极显著性。结论 牙周炎形成后牙龈和牙槽骨中PGE2 含量明显增高 ;HBO暴露后牙龈和牙槽骨中的PGE2 含量均明显降低 ,且牙槽骨中PGE2

Objective To study the effects and the therapeutic mechanism of hyperbaric oxygen (HBO) on prostaglandin E 2 (PGE 2) in alveolar bone and gingiva of experimental periodontitis in animal. Methods Experimental periodontitis was produced by silk thread sutures combined with high content sugar diet. For HBO therapy, they were exposed to a pressure of 0 25 MPa (2 5ATA), breathing pure oxygen one session a day for 60 min. The treatment course was 2 weeks. The value of PGE 2 in gingiva and alveolar bone was analyzed by enzyme immunoassay (EIA). Results The value of PGE 2 in gingiva of control group was 3 21 ng/g, and that of PGE 2 in alveolar bone was 3 22 ng/g. The contents of PGE 2 in gingiva (13 96 ng/g) and alveolar bone (13 32 ng/g) of periodontitis group increased markedly than control group ( P < 0 01). The contents of PGE 2 in gingiva (5 21 ng/g) of HBO group were 62 7% which was lower than that of periodontitis group, and the value of PGE 2 in alveolar bone (4 05 ng/g) were 69 6% lower than that of periodontitis group. The difference of PGE 2 in gingiva or alveolar bone was significant for the HBO group and periodontitis group( P < 0 01). Conclusions The contents of PGE 2 in alveolar bone and gingiva increased markedly when experimental periodontitis has formed. The value of PGE 2 in alveolar bone and gingiva reduce markedly after HBO exposure, and the decreased rate of PGE 2 in alveolar bone is more evident than that of PGE 2 in gingiva after HBO therapy.