摘要
为研究calbindinD 2 8K(CB)是否与内脏伤害性信息的传递或调控有关 ,应用免疫组织化学双重标记技术 ,对给予内脏伤害性刺激后大鼠脑干内表达Fos蛋白的CB免疫阳性神经元分布进行了观察。结果显示 :在孤束核 (NTS)、延髓腹外侧区 (VLM)、蓝斑 (LC)、臂旁外侧核 (LPB)、中脑导水管周围灰质腹外侧区 (vlPAG)等核团内均可见Fos/CB双标记神经元。双标记神经元分别占上述核团内Fos蛋白免疫阳性神经元数量的比例为12 .8% ,4 2 .7% ,4 8.1% ,14 .0 %和 13.9% ;占CB免疫标记阳性神经元数量的比例为 14 .3% ,2 4 .3% ,38.4 % ,6 .8%和 8.9%。研究结果提示 ,CB可能参与脑干内内脏伤害性信息的传递或调控。
In order to study whether calbindin D-28K (CB) is involved in the transmission or modulation of visceral nociceptive information, an immunocytochemical double labeling technique to examine the CB positive neurons expressing Fos protein induced by injecting formalin into rat stomach in the brainstem was employed. In the nucleus tractus solitarii (NTS), ventrolateral medulla (VLM), locus ceruleus (LC), lateral parabrachial nucleus (LPB) and ventral lateral midbrain peraqueductal gray (vlPAG) about 12.8%, 42.7%, 48.1%, 14.0% and 13.9% of Fos immunoreactive neurons showed CB immunoreactivity,14.3%, 24.3%, 38.4%, 6.8% and 8.9% of CB immunoreactive neurons expressed Fos protein respectively. The results of the present study suggest that the neurons containing CB might be involved in the transmission or modulation of visceral nociceptive information in the rat brainstem. \[
