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妊娠中肾细胞因子mRNA在人食管癌组织中的表达 被引量：2

Differential expression of midkine mRNA in human oesophagus cancer tissue
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摘要 目的　研究妊娠中肾细胞因子 (midkine ,MK)mRNA在食管癌组织中的表达。方法　用Trizol提取食管癌组织和相应癌旁正常组织RNA ,经RT PCR获得扩增的MKcDNA ,溴化乙锭琼脂糖凝胶电泳检测PCR产物。结果　 6例食管癌组织在45 0bp处均出现一条MK的特征条带 ,其中 1例低分化食管癌MK条带最深。 6例癌旁正常组织均检测不出MKmRNA。此外 ,2例食管癌组织除显示MK的特征条带外 ,在 2 80bp处出现一条截短型MK(tMK)区带。 结论　MK在食管癌组织特异表达 ,表达程度可能与细胞分化程度相关。 Objective To investigate the expression of midkine(MK)mRNA in human oesophagus cancer tissue.Methods The expression of MK was detected by reverse transcription-polymerase chain reaction(RT-PCR)in six surgically removed specimens of human oesophagus cancer and corresponding non-cancerous regions.Results A specific 450bp band of MK was detected in all of the six cancerous tissues in which the broadest band was in an oesophagus tissues.In addition,a 280bp band of turncated MKwas also detected in two oesophagus cancer tissues.Couclusion The results verified the specificion express of MK gene in oesophagus cancer tissues,and the degree of MK expression may be related with the severity of oesophagus cancer.
作者 张春妮 万建美 李克 马百坤 强宏娟 孟奎 韩晓冬 侯亚义
机构地区 南京军区南京总医院全军医学检验中心 南京军区南京总医院病理科 南京大学医学院
出处 《临床检验杂志》 CAS CSCD 北大核心 2002年第3期137-138,共2页 Chinese Journal of Clinical Laboratory Science
关键词 食管癌 妊娠中肾细胞因子 基因表达 MK Oesophagus cancer Midkine Truncated midkne Gene expression
分类号 R735.1 [医药卫生—肿瘤] R446.8 [医药卫生—诊断学]
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参考文献8

  • 1[1]Kadomatsu K, Tomomura M, Muramatsu T. cDNA cloning and sequencing of new gene intensely expressed in early differentiation stages of embryinal carcinoma cells and in mid-gestation period of mouse embryogenesis[J]. Biochem Biophys Res Commun, 1988,151: 1312-1318.
  • 2[2]Michikawa M, Kikuchi S, Muramatsu H, et al. Retinoic acid responsive gene product, midkine, has neurotrophic function for mouse spinal cord and dorsal root ganglion neurons in culture[J]. J Neurosci Res, 1993,35: 530-539.
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同被引文献19

  • 1王琼,赵永年,刘钧,朱治键.中期因子在食管癌中的表达及其意义[J].肿瘤研究与临床,2004,16(5):292-293. 被引量：9
  • 2王琼,赵永年,刘钧,朱治键.Midkine在食管鳞癌中的表达及其与微血管密度的关系[J].肿瘤防治研究,2006,33(1):15-16. 被引量：7
  • 3胡俊,田小强,商延芳,刘飞,刘全俊,黄培林.Midkine mRNA在食管鳞癌患者外周血中的表达及其临床意义[J].东南大学学报（医学版）,2007,26(3):181-184. 被引量：2
  • 4Kadomatsu K, Tomomura M, Muramatsu T. cDNA cloning and sequencing of a new gene intensity expressed in early differentiation stages of embryonal carcinoma cells and in midgestation period of mouse embryogenesis[J]. Biochem Biophys Res Commun, 1988, 15(3):1312-1318.
  • 5Michikawa M, Kikuchi S, Muramatsu H, et al. Retinoic acid responsive gene product, midkine, has neurotrophic function for mouse spinal cord and dorsal root ganglion neurons in culture[J].J Neurosci Res, 1993,35:530-539.
  • 6Tsutsui J, Kadomatsu K, Matsubara S, et al. A new family of heparin-binding growth differentiation factors: increased midkine expression in Wilm's tumor and other human carcinomas [J].Cancer Res, 1993, 53: 1281-1285.
  • 7Aridome K, Tsutsui J, Takao S, et al. Increased midkine gene expression in human gastrointestinal cancers [J]. Jpn J Cancer Res, 1995, 86: 655-661.
  • 8Konishi N, Nakamura M, Nakaoka S, et al. lmmunohistochemical analysis of midkine expression in human prostate carcinoma [J].Oncology, 1999, 57: 253-257.
  • 9Ikematsu S, Yano A, Aridome K, et al. Serum midkine levels are increased in patients with various types of carcinomas [J]. Br J Cancer, 2000, 83:701-706.
  • 10钟锡明,吴莺,范钰.槲皮素对食管癌细胞侵袭和中期生长因子基因表达的影响[J].世界华人消化杂志,2008,16(22):2503-2506. 被引量：5

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临床检验杂志
2002年 第3期

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