RP-HPLC法测定人血浆中美洛昔康浓度

RP - HPLC Determination of Meloxicam in Human Plasma

目的:建立RP-HPLC法测定人血浆中美洛昔康浓度。方法:采用Symmetry C_(18)分析柱(150mm×3.9 mm,5μm),以甲醇-20mmo·L^(-1)磷酸二氢钠溶液(52:48;磷酸调pH至5.20)为流动相,流速1.2mL·min^(-1)。血浆样品以卡马西平作内标,酸化后用氯仿提取并浓缩进样,检测波长为271nm。结果:标准曲线线性范围为0.02～2.0 mg·L^(-1),血浆美洛昔康萃取回收率为87.6％～92.9％,加样回收率为97.0％～99.4％,日内RSD为1.3％～1.8％,日间RSD为3.4％～5.8％。结论:本法简便,准确,重现性好,用于美洛昔康人体药动学研究血药浓度测定取得良好结果。

Objective: A simple RP - HPLC method was established for the determination of meloxicam in human plasma. Method: The chromatographic separation carried out on Symmetry C18 column (150 mm × 3. 9 mm, 5 μm) with a mobile phase consisting of methanol and 20 mmol · L-1 phosphate buffer (52:48, adjust pH to 5. 20 with H3PO4) , flow rate at 1. 2 mL ·min-1. The acidified plasma samples were extracted by chloroform with car-bamazepine used as an internai Standard. The detection wavelength was at 271 nm. Results: The calibration curve was linear within 0. 02 - 2. 0 mg ·L-1 . The extraction recoveries of meloxicam were 87. 6 % - 92. 9 %. The recoveries of methodology were 97. 0 % -99. 4 % . Intra - day RSD and inter - day RSD were 1. 3 % -1.8 % and 3.4 % - 5. 8 % respectively. Conclusion: This method is simple, accurate and reproducible. It has been applied successfully to the determination of meloxicam in human plasma for pharmacokinetic studies.