乳猪肝细胞的聚集培养术

Large-scale cultivation of porcine hepatocyte spheroids

目的 建立乳猪肝细胞与非实质细胞大量聚集培养技术。探讨获取大量高活性乳猪肝细胞的培养方法。方法 采用磁力悬浮培养技术和装置进行聚集培养。观察聚集体的形态 ,受损伤程度和白蛋白分泌功能 ,氨转化功能。结果 一次培养肝细胞数可达 2× 10 10 ～ 5× 10 10 个 ,聚集体肝细胞结构完整 ,相互之间形成胆管样结构等肝组织特异结构 ,保持良好生物活性。结论 聚集培养技术将细胞接种浓度提高 5倍 ,一次可制备足够数量的肝细胞 。

Objective To establish a method of cultivation large scale liver parenchyma and noparenchyma cells and to form the porcine hepatocyte spheroids. Methods We performed spheroid in 1 L magnetic culture vessels. Results We obtained approximately 2×10 10 ～5×10 10 hepatocyte spheroids one time and observe the hepatic special structure in hepatocyte spheroids in a electric microscope. They expressed three times more albumin secretion and twice the ammonium removal as compared with conventional monolayers. Conclusions The method of cultivating large scale liver parenchyma and noparenchyma cells of hepatocyte spheroids can provide enough hepatocyts one time for bioartificial liver support systems.