血红素氧合酶在肝硬化高动力循环中的作用

Role of Heme Oxygenase in Hyperdynamic Circulation in Cirrhosis

目的 使用血红素氧合酶抑制剂 ,探讨血红素氧合酶 一氧化碳 (HO CO)系统在大鼠肝硬化高动力循环中的作用。方法 CCl4 肝硬化模型及正常对照分别给予锌原卟啉IX(ZnPP)、NS后 ,用插管法测定血压、心率及门静脉压力。结果 与正常对照组相比 ,肝硬化组门静脉压力 (PP)明显升高 (P <0 .0 1 ) ,平均动脉压 (MAP)明显减低 (P <0 .0 1 )。ZnPP可部分纠正肝硬化大鼠的低MAP ,使PP下降 ,而对正常大鼠作用不明显。结论 HO CO系统可能是引起肝硬化高动力循环发生的重要因素之一。

Purpose: To explore the role of heme oxygenase (HO) and carbon monoxide (CO) in hyperdynamic circulation of cirrhotic rats. Methods: Liver cirrhosis was induced by percutaneous administration of carbon tetrachloride. A single dose of zinc protoporphyrin IX (ZnPP) was injected into the s. c. tissue of the posterior neck (20 μmol/kg BW) of rat. Mean arterial pressure (MAP, kPa), heart rate (HR, beats/min) and portal pressure (PP, cmH2O) were measured by indwelling catheters. Results: Typical features of cirrhosis were histologically observed in treated animals. The cirrhotic group presented a significant increase in PP [(16.71±0.78) vs (8.79±0.34)cmH 2O, P<0.011 and a significant decrease in MAP[(15.55 ± 1.67) vs (18.86±0.89)kPa, P<0.011 in comparison with the control. No-differences were observed regarding HR. Percutaneous injection of ZnPP, a specific HO inhibitor, demonstrated significantly increased MAP and significantly decreased PP in cirrhotic rats. Conclusions: HO and CO may play a role in hyperdynamic circulation of cirrhotic rats.