摘要
目的:建立牛黄息炎痛胶囊中胆红素的含量测定方法。方法:采用高效液相色谱法,Hypersil C_(18)柱(4.6mm×250mm,10μm),Nova-Pak C_(18)预柱,以二甲基亚砜-乙腈-0.5mol·L^(-1)醋酸铵缓冲液(用冰醋酸调pH=5.3)(6:6:7)为流动相,流速1.0mL·min^(-1) ,检测波长452nm。结果:胆红素在0.28~4.5μg范围内呈良好的线性关系(r=0.99999),平均回收率(n=3)分别为99.1%(RSD=1.0%);98.9%(RSD=1.3%);98.2%(RSD=1.2%)。结论:制定的含量测定方法准确,快速,有效。
Objective: To establish HPLC method for the determination of bilirubin in Niuhuang Xiyantong capsules. Method: Bilirubin in the capsules was assayed by RP - HPLC with a Hypersil C18column (4. 6mm ×250 mm, 10 μm) and a Nova - Pak C18 pre - column. The mobile phase was consisted of acetonitrile - dimethy sulphoxide-buffer containing ammonium acetate 0. 5 mol · L-1 (adjust pH to 5. 3 with glacial acetic acid) (6: 6:7). The flow rate was 1. 0 mL · min-1. The detection wavelength was 452 nm. Results: The linear range of bilirubin was 0. 28 - 4. 5 μg ( r = 0. 999 99). The average recoveries ( n = 3 ) were 99. l % ( RSD = 1.0%), 98.9% (RSD = 1.3%), 98.2% (RSD = 1.2%) respectively. Conclusion: The method is rapid, simple and accurate.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2002年第3期198-200,共3页
Chinese Journal of Pharmaceutical Analysis