彗星系统定量检测柠檬醛损伤黄曲霉DNA的研究

Study on Quantitative Test on the DNA Damage of Aspergillus flavusCaused by Citral with a Comet Analysis System

理化因素致细胞DNA损伤 ,彗星测试提供了一个直观的方法。采用新型SCGE图象分析系统 (IMI1 0 ) ,将细胞显微分光光度分析与显微成像及图象分析结合 ,直接检测柠檬醛致黄曲霉核DNA损伤 ,与国际流行的SCGE图象分析系统相比 ,具有分析速度快、便于分析 ,同时具有中英文可切换界面和多格式输出打印特点。该系统使彗星试验的检测时间缩短 2 3 ,并提高了准确性 ,可实现对活细胞多种结构参数、细胞内分子与膜的变化状况同时进行长时间连续的动态瞬间监测 。

Comet assay provided a object method for DNA damage caused by physical and chemical factors.A new system of SCGE image analysis,which combines microphotodensitometry and microphotography with image analysis have been developed.Compared with the international popular,it has well at speed,convenience,exchange face of Chinese and English and print.We used it to directly determine the A.flavus' DNA damages for citral.The results show that test time was shorted 2/3 and accuracy was improved.It is probable that cell structural parameter,cell molecule and membrane could be test long,successive,movably and instantly.So the new system has a wide application.