应用Coriolus vericolor菌丝球脱色染料及印染废水的研究

Decolorization of Dyestuff and Dying Waste Water by Laccase Solution with Self-flocculent Mycelial Pellets of Coriolus versicolor

对白腐真菌 (Coriolusvericolor)产生漆酶进行了研究。发现该菌产漆酶的最适初始pH值为 4 5。提高微量元素浓度或添加藜芦醇都可使C .versicolor的产酶能力增加 ,添加Tween80会有一定的抑制作用。采用C .versicolor菌丝球进行重复分批产酶试验 ,结果表明菌丝球的稳定性很好 ,同一批菌丝球可连续利用 1 4次 ,平均每批酶活力可保持在 6 72U mL ,产酶能力优于聚氨酯泡沫固定化菌丝。将粗酶液用于染料的脱色降解试验 ,在酶活力为 3 3IU mL ,酸性橙浓度为 5 0 0mg L条件下 ,经过 2 4h反应 ,脱色率达到 98 5 % ;对含弱酸大红和卡布龙红的印染废水进行脱色试验 ,脱色率也达到了 93 %。

Both laccase production by the white-rot fungus Coriolus versicolor and decolorization of dyestuff and dying waste water with crude solution of laccase were studied in this work. Laccase production meets the definition of secondary metabolism. For laccase production the optimum initial pH is 4.5. Addition of veratryl alcohol or elevated trace metals could both enhance the laccase activity, while Tween80 showed some inhibition. The immobilized mycelia of C.versicolor in polyurethane foam had less laccase production ability than mycelial pellets. A repeated batch cultivation process was found to be a very economical way for laccase harvest. The same pellets could be used for at least 14 times and average laccase activity of each batch could maintain 6.72 IU/mL. This method reduces the enzyme production course, medium consumption and the possibility of contamination, showing high efficient and great economic benefit. Good results were also obtained in decolorization experiments with the crude solution of laccase. With 3.3IU/mL initial laccase activty, color removal of Acid Orange reached 98.5% after 24h reaction. Also with 2.6IU/mL initial laccase activity, color removal of dying waste water reached 93% after 24h reaction.