不同温度条件对下丘脑神经元K^+单离子通道簇状开放的影响

EFFECTS OF TEMPERATURE ON THE BURST OPENING OF VOLTAGE-DEPENDENT K^+ CHANNELS IN HYPOTHALAMIC NEURONS

目的 :探讨温度对下丘脑神经元电压依赖性K+ 单离子通道 (Kv)簇状开放 (Burstopening)的影响。 方法 :采用膜片钳细胞贴附式技术观察和记录 32℃、37℃和 39℃时下丘脑神经元Kv的活动。结果 :温度升高 ,单位时间内记录到的Burst开放明显增多 ,平均开放间期变长。当温度从 32℃升至 39℃ ,B1从 1.5ms升至 8.1ms ,B2 从 6 .6ms升至 83.2ms,Burst内部开放数目也由 1～ 2个升至 8个 ,从以简单Burst开放 (SB)为主转变成以复杂Burst开放(CB)占优势。结论 :温度升高 ,下丘脑神经元上IK 通道更多地处于活动状态 ,这可能有利于下丘脑神经元对体温变化的调节。

Aim and Methods: To observe the effect of temperature on burst opening of voltage dependent K + channels(Kv) in hypothalamic neurons by cell attached mode of patch clamp technique. Results: With temperature raising, the number of burst opening increased, so did its average burst duration. B 1 and B 2 raised from 1.5 ms and 6.6 ms at 32℃ to 8.1 ms and 83.2 ms ( P <0.05) respectively while the open number of inter burst, from 1～2 to 8 ( P <0.05) too. Instead of SB, CB displayed predominantly a kind of burst opening. Conclusion: More burst opening of Kv in hypothalamic neurons with temperature raising, this was benefited to the body temperature regulation of neurons on hypothalamus.