维生素E对应激大鼠海马齿状回长时程增强的保护作用

THE PROTECTION OF VITAMIN E ON LTP IN HIPPOCAMPAL DENTATE GYRUS OF RATS UNDER STRESS

目的 :探讨维生素E对应激大鼠海马齿状回长时程增强的保护作用。方法 :在束缚应激条件下 ,通过补充维生素E(VE) ,观察大鼠在应激过程中的行为效应。结果 :接受应激大鼠在旷场实验中的穿行格数明显增加 ;长时程增强 (LTP)诱发率降低 ,突触传递功能减弱 ;血浆糖皮质激素水平明显升高。而应激同时适量补充VE的大鼠未出现上述异常变化。结论 :适当补充维生素E可减轻应激性海马突触传递功能障碍 。

Aim: To study the effects of vitamin E on stress induced impairments in hippocampus of rats. Methods: Twenty four male Wistar rats were randomly allocated into four groups: control, stress, control+VE, stress+VE. The rat stress model was built by restraining for 6 h/d,21 d. The long term potentiation was induced in rat hippocampal dentate gyrus(DG) by high frequency test stimulation. Results: Compared with control group, the rats suffered from restraint stress showed that the number of crossing in open field test and the content of glucocorticoids in plasma was significiantly increased, the changes of amplitude of population spike(PS) were significiantly lower. After VE supplementation in stress rats, the indices mentioned above were significiantly improved. Conclusion: Opportune supplementation of vitamin E may improve the brain function under stress.