摘要
目的 :探讨白细胞介素 2 (1L 2 )对心脏节律作用及其可能机制。方法 :采用体外培养乳鼠心肌细胞模型和离体人鼠灌流心脏模型 ,观察培养的心肌细胞搏动频率和离体心脏心率及节律。结果 :① 2 .5～ 2 0 0u/ml的IL 2呈浓度依赖性地降低心肌细胞的搏动频率。② 5 0u/ml的IL 2明显增加离体心脏心率和室性早搏个数。③propranolol预处理可取消 5 0u/mlIL 2的离体心脏作用。④热失活IL 2对培养的心肌细胞搏动频率和离体心脏心率和心律都无显著作用。结论 :IL 2可直接抑制培养心肌自律性 ,其对离体心脏的正性变时和致心律失常作用可能由内源性儿茶酚胺介导。
Aim: To explore the effect and mechanism of interleukin 2 (IL 2) on the rhythm of isolated rat heart and neonatal myocytes. Methods: Cultured neonatal rat cardiomyocytes and isolated perfused rat heart were used. Results:① IL 2 (2.5～200 u/ml) reduced the spontaneous beating rate of cultured neonatal rat cardiomyocytes in a dose dependent manner. ② IL 2 at 50 u/ml increased both the heart rate and the number of premature ventricular excitation in the isolated heart. ③ Pretreatment with propranolol abolished the effect of 50 u/ml IL 2 on the isolated heart. ④Heat inactivated IL 2 had no significant effect on the cultured cardiomyocytes and isolated hearts. Conclusion: IL 2 inhibited the autorhythmicity of cultured cardiomyocyte directly while the positive chronotropic and arrhythmogenic effects of IL 2 in the isolated rat heart may be mediated by endogenous catecholamine.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2002年第2期149-151,共3页
Chinese Journal of Applied Physiology
基金
浙江省自然科学基金青年人才专项资金资助(RC990 3 8)
医学院中青年科研起动基金资助
