含抑肽酶灌注液减轻缺血再灌注心肌的损伤

Aortic perfusion solution containing aprotinin reduce reperfusion myocardium injury during myocardial ischemia

目的 观察抑肽酶等药物对缺血再灌注离体兔心的影响。方法 2 4只家兔随机分为两组 :对照组 (B组 )将离体心脏置于 L angendorfl装置 ,经主动脉逆行灌注克氏液 ,灌注 30 min后 ,用 Thomas灌注停跳 40 min,最后再恢复灌注 30 min。用药组 (A组 )克氏液中加入抑肽酶及山莨菪碱。缺血前及再灌注期记录左心室功能及冠状动脉血管阻力 ,并行心肌超微结构观察。结果 再灌注后 30 min,A组左心室功能明显高于 B组 (P<0 .0 5 ) ;收集的灌注液中肌酸磷酸激酶 (CK)及乳酸脱氢酶 (L DH)较 B组明显减少 ;超微结构改善也优于 B组。

Objective To observe the effect of aprotinin on isolated rabbit hearts from ischemia-reperfusion injury. Method Isolated contragrade perfused left-side working heart model was established.Hearts of ischemic arrest was induced by cardioplegia was exposed to 40 minutes.Before and after ischemia hearts were perfused with either aprotinin(group A)K-H solution or normal K-H solution(group B)for 30 minutes.Cardiac hemodynamic parameters were measured before ischemia and period of reperfusion. Myocardical creatine kinase (CK) and lactate dehydroge- nase(LDH) were measured. Myocardical ultrastructure was observed after reperfusion. Rsult The recovery of cardiac function (group A) and the higher coronary flow were significiently better than that of group B (P<0.05),CK and LDH levels in group A were lower than that in group B(P<0.05); improvement of myocardial ultrastructrue after reperfusion in group A was superior to that in group B. Conclusion Aprotinin signifcantly protect myocardial function from reperfusion injury during myocardial ischemia.