PPAR-γ在溃疡性结肠炎粘膜中的表达

The expression of PPAR-γin colonic mucosa of paitent with ulcerative colitis

目的了解过氧化物酶增生激活受体（ＰＰＡＲ－γ）在溃疡性结肠炎（ＵＣ）患者结肠活检粘膜中的表达及其与疾病严重度的关系。方法 采用免疫组化法测定３１例ＵＣ患者和１２例对照组的结肠活检粘膜中ＰＰＡＲ－γ的表达情况。结果 ＵＣ患者受累及相对正常的结肠上皮ＰＰＡＲ－γ的表达均较正常对照组下降，三组分别为２４．７％±１．４９％、４３．８％±２．０３％、７０．２％±３．７５％（Ｐ＜０．０５），且病变越重，表达越低。结论ＵＣ患者内镜下活检粘膜中ＰＰＡＲ－γ的表达下降，并与疾病严重程度成负相关，提示其可能与ＵＣ的发病有关。

Objective To examine the expression of PPAR-γ in the biopsy of colonic mucosa of patients with ulcerative colitis(UC) and assess its relationship with the seriousness of disease .Methods The biopsy specimens were taken endoscopically from 31 patients with ulcerative colitis (UC) and from 12 IBS control separately. The expression of PPAR-γ antigen in colonic mucosa was detected by immunohistochemisty(IHC) of the labeled-avidin-biotin method .The first antibody was mouse to human monoclonal IgG antibody against PPAR-γ.Results The percentages of positive cells in the lesion of UC its juxta mucosa and control were 24.7%±1.4 9%,43.8%±2.03% and 70.2%±3.75%(P<0.05)respectively.The expression in UC was lower than that in control. The expression of positive cells in three grades (mild?moderate?severe) of UC were 43.4%±3.67%,34.4%±3.35% and 10.3% ±1.42% respectively.The higher the grade of inflammation,the lower the expression. Conclusion The mucosal expression of PPAR-γ was decreased in UC and was adversely related to the seriousness of UC.The defecit in PPAR-γ maybe involved in the pathogenesis of UC.