光敏核不育水稻育性转变敏感期超氧化物歧化酶与丙二醛的变化

THE DIFFERENCES OF SUPEROXIDE DISMUTASE ACTIVITY AND MALONDIALDEHYDE CONCENTRATION DURING THE PHOTOPERIOD SENSITIVE STAGE OF FERTILITY CHANGES IN HPGMR

分析了湖北光敏核不育水稻农垦58s(简称58s)和对照农垦58(简称58)在长日照(LD)、短日照(SD)下,其幼穗发育期叶片超氧化物歧化酶(SOD)、过氧化氢酶、过氧化物酶活性与幼穗丙二醛(MDA)的含量及花粉的育性。结果表明在光照诱导育性转变敏感期58s(LD)其 SOD 活性高于其他3个处理[58s(SD)、58(LD)、58(SD)],且活性变化的时相亦不同于其他3个处理;58s(LD)其幼穗 MDA 含量亦远高于其他3个处理,显示其膜脂质过氧化程度高于其他3个处理,提示58s 在育性转变敏感期受长日照诱导不育与自由基伤害有关。

The activity of superoxide dismutase(SOD),catalase and peroxidase of leaves and concentration of malondialdehyde(MDA)of young panicles as well as the fertility of pollens were determined in different developing stages of young panicles under the experimental conditions of long day 16 hours(LD)light and short day 10 hours(SD)light with two rice varieties.HPGMR(i. e. Hubei Photoperiod Sensitive Genie Male-Sterile Rice)Nongken 58s and Nongken 58. The main results are summarized as follows: During the photoperiod Sensitive stage of fertility change(i. e. the stage from differentiation of secondary branch primordium to formation of pollen mother cell),the SOD activity of HPGMR Nongken 58s(LD)was higher than that of the other three sampl[i. e. HPGMR Nongken 58s(SD),Nongken 58 (LD)and Nongken 58(SD)]and the phase of SOD activity change of HPGMR Nongken 58s(LD)was also different from the other three samples.The contents of MDA in HPGMR Nongken 58s(LD)was higher than that in the samples as mentioned above. The increase of MDA produced by lipid peroxidation could reflect the accumulation of oxygen free radical toxicity.Thus,oxygen free radical'may play an important role in the male sterility of HPGMRNongken 58S(LD).