干扰素-α抗鼠肝纤维化的作用

Experimental study of the therapeutic effect of interferon-α on liver fibrosis in rats

目的 观察干扰素 α 2a(IFN α 2a)对实验性肝纤维化时肝脏组织学及血清肝纤维化指标的影响。方法 利用免疫组织化学、放射免疫技术及MV1LU细胞生长抑制法 ,对CCl4诱导大鼠肝纤维化不同阶段肝脏组织学及透明质酸 (HA)、层黏蛋白 (LN)、Ⅳ胶原 (ⅣC)及转化生长因子 β(TGF β)的变化进行了观察。 结果 在肝纤维化早期 (3周 ) ,干扰素 α治疗组肝脏炎症计分、纤维化计分及血清纤维化指标与模型对照组比较差异无显著性 (P >0 .0 5 )。在肝纤维化中期 (6周 )及晚期(12周 ) ,IFN α 2a治疗组组织学肝脏炎症计分、纤维化计分与模型对照组对比差异有显著性 (P <0 .0 5及 <0 .0 1)。血清TGF β与HA、LN、ⅣC呈正相关 ,TGF β活性与肝纤维化动态变化相一致。 结论 干扰素 α 2a可明显减轻肝脏炎症 ,减少或逆转肝纤维化的作用 ,其机制与抑制肝脏中TGF β的活性有关。

Objective To observe the effects of interferon α on the histopathology and the levels of serum fibrosis markers in experimental rat liver fibrosis. Methods Dynamic changes and relationships among liver histopathology and serum HA, LN, ⅣC, TGF β in fibrotic liver induced by CCl 4 were studied using immunohistochemistry, RIA techniques and modified MV1LU cell growth inhibitory assay. Results At the 3rd week after fibrotic livers induced by CCl 4, the liver histopathology and serum levels of HA, LN, ⅣC, TGF β in interferon α treated group showed no difference from those in control group( P > 0.05 ), However, all pasameters showed significantly milder or lower in interferon α treated group than incontrol group at the 6th, 12th week( P <0.05 or <0.01) respectively. The level of serum TGF β was positively correlated with those of HA, LN, ⅣC and the staging of fibrotic liver. Conclusions Interfe ron α is effective in attenuating liver fibrosis by down regulating the level of serum TGF β.