狼疮性肾病患者与红细胞补体受体Ⅰ型分子基因型相关性研究

The research about gene type of red blood cell complement receptor 1 (CR_1) in lupus nephritis

采用 PCR和 Hind 酶切技术以及酶联法定量测定狼疮性肾炎 (L N)患者、非 L N患者及正常人的红细胞补体受体一型分子 (CR1 )数量。结果示 L N患者红细胞 CR1 分子密度相关基因型高表达较非 L N患者及正常人明显下降 ,中、低表达明显升高 (P<0 .0 5～ 0 .0 1) ;L N患者 CR1 分子的数量表达全部低于非 L N患者及正常人平均水平 (P<0 .0 0 1) ,并且 CR1 分子数量表达的变化与病情活动度呈明显相关。认为 :对 L N患者进行红细胞CR1 分子定量测定 ,对临床诊断和病情判定具有重要参考价值 ,对探讨 L N病因也有很大的启示。

Detection of RBC CR1 molecule quantity are used by PCR,Hind Ⅲ edonuclease techniques in LN,non-LN and control.High expression gene type in LN patients' RBC is lower than that in non-LN or control,But moderate and low expression types in LN are higher than that in control(P<0.05～0.01).Quantity of CR_1 molecule in LN patients is lower than that of average level in non-LN and normal person(P<0.001).The detection of CR_1 molecule quantity in LN patients' RBC is beneficial to clinical diagnosis and prognosis.