原位RTPCR测定雄性幼鼠肾上腺中Ⅰ、Ⅲ型17β羟甾脱氢酶的表达

THE EXPRESSION OF 17β HSD TYPE Ⅰ AND Ⅲ IN IMMATURE MOUSE ADRENAL GLAND

目的 探讨生后 5d小鼠肾上腺X带细胞的功能及其与雄性性分化的关系。 方法 应用原位RT PCR技术分别测定 5d小鼠肾上腺中Ⅰ、Ⅲ两型 17βHSD的表达。 结果 在肾上腺皮、髓质交界的X带细胞中有Ⅰ、Ⅲ两型 17βHSD的表达。 结论 未成年小鼠肾上腺X带细胞能合成雄激素 ,并与睾丸合成的雄激素共同促进雄性性分化。

Objective To study the function of the X\|zone cells in the 5 days old male mouse adrenal gland and the relationship between the function and the sexual differentiation. Methods The technique of in situ RP\|PCR was used to detect the expression of type Ⅰ and Ⅲ 17β HSD mRNA. Results In the cells of X\|zone which located in the interface between adrenal crotex and medulla, there were expressions of type Ⅰ and Ⅲ 17β HSD.Conclusion\ The X\|zone cells of adrenal gland in immature mouse could synthesize androgen, enhanced the male sexual differentiation together with the androgen synthesized from the testis.\;[