辣根过氧化物酶标记的纯视细胞层移植

HORSE-RADISH PEROXIDASE HRP-LABELING PHOTORECEPTOR CELL TRANSPLANTATION

目的 利用HRP标记Wistar鼠的视细胞 ,观察移植的纯视细胞在受体的分布。 方法 30 %的HRP标记Wistar鼠视细胞 ,经外路途径移入皇家外科学院鼠 (RoyalCollegeofSurgeonRat,RCS)的视网膜下腔 ,术后 2周取RCS鼠眼做冰冻切片 ,组织化学染色法染色 ,普通光学显微镜下观察。 结果 在视细胞移植术后 2周 ,移植视细胞存活 ,HRP标记的Wistar鼠纯视细胞层在RCS鼠视网膜内呈褐色 ,排列平覆。

Objective We observe the grafted Wistar rat's photoreceptor cell labeled by Horse\|radish peroxidase (HRP) in the receptor. Methods The healthy Wistar rat's photoreceptor cells were labeled and grafted into the sub\|retinal space of the left eye of the Royal College of Surgeon (RCS) rat by outer\|path way. Two weeks later, the eyes of the RCS rats were stained by cyto\|chemistry method and processed for light microscopic. Results After two weeks of transplantation, the transplanted photoreceptor cells survived. HRP\|labeling photoreceptor cell was stained brown and ranged orderly.Conclusion\ These results suggest that we can observe the grafted Wistar rat's photoreceptor cell in the RCS rat's retina by transplanting the HRP\|labeling photoreceptor cells.\;[