HSV-TK基因在膀胱癌小鼠体内的转移和丙氧鸟苷治疗效果

In vivo HSV-TK gene transfer and the therapeutic effects of ganciclovir on bladder cancer in mice with HSV-TK gene transfer

目的探讨HSV-TK基因在膀胱癌动物体内的转移途径,观察HSV-TK/GCV(丙氧鸟苷)系统体内治疗的效果。方法在T739同基因鼠膀胱癌皮下肿瘤模型中,以裸质粒DNA瘤内直接注射、脂质体包裹和逆转录病毒介导等3种不同方法转移HyTK基因,观察丙氧鸟苷对膀胱癌的治疗效果。结果脂质体复合物组及逆转录病毒组背部肿瘤生长减慢,抑瘤率分别为54%、68%;动物平均存活时间分别比对照组延长28.81%、44.16%。结论脂质体、逆转录病毒介导的基因转移是膀胱癌基因治疗的有效手段。

Objective To explorean effectivemethodforin vivo HSV-TKgenetransferandobservethetherapeuticeffectof ganciclovir(GCV)on bladdercancerin miceafterreceivingsuchgenetransfertherapy.Methods Micemodelsbearing subcutaneoustumorsof bladdercancerwereestablishedin24syngeneicmice,andHSV-TKgenetransferwasperformedby meansof nakedDNA plasmids,DNA-liposomecomplexor retroviralmediatorsrespectively.GCV treatmentwas administeredin allthemiceanditseffecton thegrowthof thetumorwas subsequentlyobserved.Results Genetransfer therapiesby DNA-liposomecomplexandretroviralmediatorsbothsignificantlyinhibitedthegrowthof thetumorby54%and68%respectively,andthesurvivalof themicewasprolongedby28.81%and44.16%(P<0.05).Theeffectof genetransferby nakedDNA plasmidswas not obvious(P>0.05).Conclusion Genetransfertherapiesby DNA-liposomecomplexand retroviralvectorssupplementedby GCVtreatmentareeffectivetherapeuticmodalitiesagainstbladdercancer.