幽门螺杆菌感染对猪胃粘膜上皮增生的影响

Effects of Helicobacter pylori infection on porcine gastric epithelial cell proliferation

目的探讨幽门螺杆菌(Hp)感染与猪胃粘膜上皮增生的关系。方法应用“中国1号小型猪”建立Hp相关性胃炎模型,定量检测Hp感染与增殖细胞核抗原(PCNA)、DNA倍体含量变化的相互关系。结果Hp感染阳性组(n=13)的猪胃粘膜上皮细胞PCNA标记指数(LI%)(40.95±3.6),明显高于Hp阴性对照组(n=10)的猪胃粘膜上皮细胞PCNALI%(29.41±2.82),Hp感染阳性组的猪胃粘膜上皮细胞DNA二倍体与近二倍体含量(70.78%)低于对照组(90.65%),而增殖倍体与非整倍体含量均高于对照组。结论猪胃粘膜上皮细胞增生增强可能与Hp感染有关。

Objective To investigatetheassociationbetweenporcinegastricepithelialcellproliferationand Helicobacter pylori(Hp)infection.Method Animalmodelsof gastritisassociatedwithHpinfectionwereestablishedusing'ChineseNo.1pigs'.Theexpressionsof proliferatingcellnuclearantigen(PCNA)andDNApolyploidcontentin porcinegastricepithelial cellswerequantitativelyassayedby meansof immunohistochemistryandFeulgenstainrespectively.Results Thevaluesof PCNAlabelingindex(LI)intheporcinegastricepithelialcellsof theexperimentalgroupweresignificantlyhigherthanthose of thecontrolgroup(40.95±3.60vs29.41±2.82,P<0.01).TheDNAdiploidor approximatediploidcontentinthegastric mucosaof thepigswas significantlylower(70.78%vs90.65%,P<0.01),buttheproliferationpolyploidandnon-doubleploid of DNAsignificantlyhigherintheexperimentalgroupthaninthecontrolgroup(P<0.01).Conclusion Hpinfectionmayplay a roleto enhancetheproliferationof gastricepithelialcellsinpigs.