广东汉族人群乙肝疫苗免疫应答水平与HLA-DRB1*02,07,09的相关性

Association of immune response to hepatitis B vaccine with HLA-DRB102,07,09genes in the population of Han nationality in Guangdong Province

目的研究乙型肝炎(乙肝)疫苗免疫应答水平与HLA-DRB1*02,07,09等位基因的相关性。方法对1400名广东籍汉族健康大学生进行重组乙肝疫苗标准全程接种(10μg/次,0、1、6月),末次接种后第8周用ELISA检测血清抗-HBs水平,对全部无、弱应答(共118名)、100名中等应答者(随机选取)和全部强应答者(45名)通过序列特异性引物聚合酶链反应法(PCR-SSP)进行HLA-DRB1*02,07,09基因的检测。结果无、弱应答组携带HLA-DRB1*07基因的频率为15.25%,高于中、强应答组的3.44%(P<0.05);无、弱应答组携带HLA-DRB1*02的频率为5.08%,低于中、强应答组的17.9%(P<0.05);无、弱应答组和中、强应答组携带HLA-DRB1*09的频率分别为19.49%和17.9%,二者比较无显著性差异(P>0.05)。结论HLA-DRB1*07与广州某大学汉族人群乙肝疫苗无、弱应答相关,HLA-DRB1*02与该人群乙肝疫苗中、强应答相关,HLA-DRB1*09与该人群乙肝疫苗应答水平无明确的相关性。

Objective To studytheassociationbetweenimmuneresponseto hepatitisB vaccineandHLA-DRB1*02,07,09genesinthepopulationof HannationalityinGuangdongProvince.Methods A totalof1400healthycollegestudentsof Han nationalityfrom GuangdongProvincewere recruitedin thisstudy,who receivedstandardcoursesof vaccinationwith recombinanthepatitisB virusvaccineat month0,1and6.Eightweeksafterthefinalvaccination,theserumlevelsof anti-HBsantibodywas examinedwithenzyme-linkedimmunosorbentassay(ELISA).Detectionof HLA-DRB1*02,07,09alleleswereperformedwithPCRusingsequence-specificprimers in118individualswho failedto produceor produced insufficientantibodies(nonrespondersor weakresponders),100normalrespondersand45strongresponders.Results The frequencyof HLA-DRB1*07in nonrespondersor weakresponderswas significantlyhigherthanthatin normalor strong responders(15.25%vs3.44%,P<0.05),while thefrequencyof HLA-DRB1*02was muchlower(5.08%vs17.9%,P<0.05).The frequencyof HLA-DRB1*09showedno obviousdifferencebetweenthe2groups(19.49%vs17.9%,P>0.05).Conclusion Failureor deficiencyin the responseto HBV vaccineis associatedwiththe HLA-DRB1*07gene,while HLA-DRB1*02geneis relatedto thenormalvaccinationprocessor overreaction.Definiterelationof HLA-DRB1*09to the responseto HBVvaccineisnotfound.