电离辐射对小鼠胸腺bcl-2蛋白表达及细胞凋亡的作用机制

Mechanism of effect of ionizing radiation on bcl-2 protein expression and apoptosis in mouse thymus

目的 :研究不同剂量 X射线照射对小鼠胸腺 bcl-2蛋白表达及细胞凋亡的作用机制。方法 :用免疫组织化学、图像分析技术及透射电镜技术分别检测小鼠胸腺 bcl-2蛋白表达及细胞凋亡。结果 :假照组胸腺实质内 bcl-2蛋白表达主要分布于髓质 ,胸腺皮质内可有少量凋亡的胸腺细胞。 2 Gy照射后 4、8和 1 2 h胸腺髓质内 bcl-2表达减少 ,皮质内出现许多典型的凋亡细胞 ;0 .0 75 Gy照射后 bcl-2蛋白表达增多 ,皮质内凋亡细胞减少 ,且可见较多的细胞分裂像。结论 :不同剂量电离辐射对胸腺细胞凋亡的影响与胸腺髓质内

Objective: To study the mechanism of effect of ionizing radiation in varied doses of X rays on bcl 2 express and apoptosis in mouse thymus.Methods: Immunohistochemistry, image analysis and transmission electron microscope were used in the study. Results: The expression of bcl 2 protein was limited within thymic medulla, decreased with 2 Gy, however, increased with 0.075 Gy after whole body irradiation. Some typical apoptotic cells were found in thymic cortex after 2 Gy irradiation. The apoptotic cells decreased and mitotic metaphase increased after 0.075 Gy irradiation. Conclusion: The mechanism of effect of ionizing radiation on apoptosis of thymus was related with the expression of bcl 2 proteins.