苯乙酸对胶质瘤C6细胞集落形成的作用

Effect of phenylacetate on the colony formation of glioma C6 cell line

目的 :研究诱导分化剂苯乙酸 (PA)对胶质瘤 C6细胞增殖的抑制作用 ,探讨此过程中 PA对癌基因 c-myc及抑癌基因 P1 6蛋白水平表达的影响。方法 :应用平板集落形成试验及 [3H]-Td R掺入试验检测增殖抑制 ;以免疫组化 SP染色法检测基因蛋白水平的表达。结果 :应用 PA后 ,C6细胞平板集落形成能力下降 ,PA 2 .5 mmol· L- 1 处理组及 5 .0 mmol· L- 1 处理组抑制率分别达到90 .4%和 99.1 %。同时 [3H]-Td R掺入量降低 ;c-myc基因胞浆阳性表达下降 ,阳性细胞灰度值(Y值 )对照组为 (1 3 0 .5± 2 .8) ,2 .5 mmol·L- 1处理组为 (1 5 2 .8± 3 .1 ) (P<0 .0 1 ) ;P1 6基因胞浆阳性表达升高 ,对照组 Y值为 (1 67.1± 6.2 ) ,2 .5 mmol· L- 1处理组为 (1 49.2± 1 .5 ) (P<0 .0 1 )。结论 :PA对 C6细胞增殖存在时间和剂量依赖性抑制 ,其机制可能与抑制癌基因 c-myc对增殖的转录调节并激活了抑癌基因 P1

Objective: To study the effect of phenylacetate (PA) on proliferation and the protein expression of c myc and P16 gene in glioma C6 cell line. Methods: The TdR intermingling assay was used to evaluate the effect of PA on cell proliferation. c myc and P16 protein expression were examined by immunohistochemical technique. Results: TdR intermingling assay showed that the CPM value was lower in PA group than that of control group. The carcinoma cells became well differentiated and the colony forming rate was significantly decreased. The protein expression level of c myc gene was decreased, while P16 gene was increased in PA group. Conclusion: PA caused a dose and time dependent proliferation inhibitory effect through inhibiting the expression of oncogene c myc and improving the expression of tumor suppression gene P16 protein.