肿瘤细胞中两种TNFR的表达及新型rhTNF对肿瘤细胞生长的影响

Expression of TNFRs in tumor cells and effect of novel rhTNF on tumor cell growth

目的 探讨ＴＮＦＲｐ５５和ＴＮＦＲｐ７５在各种肿瘤组织中的表达与新型ｒｈＴＮＦ对肿瘤细胞生长的抑制作用的关系。方法应用免疫组织化学染色法，观察１２０例肿瘤组织和１０株体外培养的肿瘤细胞中，ＴＮＦＲｐ５５和ＴＮＦＲｐ７５的表达及分布。同时应用结晶紫摄入法，观察ｎｒｈＴＮＦ对肿瘤细胞生长的抑制。结果 在肿瘤组织和肿瘤细胞中，ＴＮＦＲｐ５５的阳性表达率分别为８２．５％和９９％，ＴＭＦＲｐ７５分别为 ８４．２％和９８．８％，不同类型肿瘤间的表达无明显差异（Ｐ＞０．０５）．ｎｒｈＴＮＦ可抑制ＳＷ４８０、ＳＧＣ７９０１、８９１０ＳＭＭＣ７７２１和ＢＥＬ７４０２这５株肿瘤细胞生长，但是不能抑制另外５株肿瘤细胞Ａ５４９、ＰＬＡ８０１、Ｈｅｌａ、Ｅｃａ１０９和ＧＲＣ－ｌ的生长。结论ＴＮＦＲ广泛存在于各种肿瘤细胞上，ｎｒｈＴＮＦ抑制肿瘤细胞生长的程度与ＴＮＦＲｐ５５的表达率无相关性。

Aim To investigate the relationship between the expres- sion of TNFRp55 and TNFRp75 in tumor tissues and tumor cell lines in vitro and the supression of novel rhTNF on these tumor cell lines growth. Methods Expressions of TNFRp55 and TNThp75 in l20 tumor tissues and 10 tumor cell lines in vitro was evaluated by immu- nohistochemical staining. The suppression of nrhTNF on tumor cell lines growth was detected by using crystal violet staining. Results In these tumor tissues and tumor cells, the positive rates of TNFRp55 expression were 82. 5% and 99% respectively, whereas those of TNFRp75 expression was 84. 2% and 98. 8% respectively. There were no significant difference among different types of tumors(p > 0. 05). nrhTNF could inhibite SW480, SGC79O1, 8910, SMMC772I and BEL7402 cell lines growth and had no effect on A549, PLA8O1, Hela, EcalO9 and GRC- 1 cell lines. Conclusion TNFRs exist on different tumor cells. There are no correlation between nrhTNF inhibition on tumor cell lines growth and the TNFRp55 expression on these tumor cells.