摘要
目的观察地塞米松（ＤＭ）对哮喘豚鼠体外不同密度的嗜酸性粒细胞（Ｅｏｓ）凋亡及ｂｃ－２表达的影响，并探讨激素促进哮喘ＥＯＳ凋亡的机制。方法以卵蛋白激发哮喘豚鼠模型４８ｈ后，进行支气管肺泡灌洗，分离灌洗液中不同密度的Ｅｏｓ。加入ＤＭ（１０～（１０）～１０～５ｍｏｌ／Ｌ），以原位杂交法检测Ｅｏｓ的ｂｃｌ－２表达，ＴＵＮＥＬ法检测Ｅｏｓ的凋亡。结果ＤＭ干预２４ｈ，可见Ｅｏｓ凋亡增加，ｂｃｌ－２ｍＲＮＡ表达降低，且呈剂量依赖性。ｂｃｌ－２ ｍＲＮＡ的表达与Ｅｏｓ凋亡呈负相关。结论ＤＭ可抑制ｂｃｌ－２的表达，促进ＥＯＳ凋亡。ｂｃｌ－２表达的减少可能是ＤＭ调节Ｅｏｓ凋亡的机制之一。
Aim To investigate the effect of dexamethasone ( DM) on expression of bcl-2 mRNA and apoptosis of eosinophils( Eos) in bronchoalveolar lavage fluid ( BALF) from asthmatic guinea pig, and mechanism of Eos apoptosis promoted by steroid. Methods After giunea pig were stimulated with ovalbumin (OVA) for 48 hours, hypodense Eos ( HEos) and normodense Eos ( NEos) were i- solated from BALE by gradient centrifugation with percoll. The Eos cultured in RPMI1640 medium. DM ( 10^(-10)~10 ^(-5) was added to the wells. The expression of bcl -2 mRNA in Los and apoptosis of Eos was detected by in situ hybridization and TdT- mediated dUTP nick end labeling( TUNEL), respectively. Results After 24 hours Culture in the presence of DM, apoptotic Los increased and expres- sion of the bc 1 - 2 mRNA decreased in a dose- dependent manner. The expression of bcl -2 mRNA was correlated negatively with Eos apoptosis. Conclusion DM may promote Eos apoptosis and inhibit expression of bc 1-2 in dose - dependent manner in vitro. Inhibition of bc 1-2 expression might be one of the mechanisms of Eos apopto- sis promoted by DM.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2002年第1期16-18,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助
No.39670337
