特异性脱颗粒肥大细胞的形态学研究

Study on the morphological character of specific degranulated mast cells

目的 观察特异性脱颗粒肥大细胞的形态特征。方法 通过抗ＩｇＥ单抗（抗ＩｇＥ ｍＡｂ，ＨＲＰ）与已致敏的肥大细胞相结合，用ＤＡＢ加中胜红双染色法对肥大细胞进行染色。结果 发现无论肥大细胞是否脱颗粒，只有被ＩｇＥ致敏的肥大细胞才能被染成棕褐色；而非特异性（即未被ＩｇＥ致敏的肥大细胞），无论其脱颗粒与否，都呈现中性红的颜色，即胞浆内呈现砖红色的颗粒。结论 该实验为评价患者当前所处状态（是高敏体质还是非高敏体质？是疾病缓解期抑或发作期等？）、预测哮喘等Ⅰ型变态反应的病程转归、判断疗效，以及进行一些与肥大细胞脱颗粒有关的科学实验，提供了一种新的更客观的方法。

Aim To observe the morphological charaeler of specific rip- granulated masl cells. Methods The sensitized mast cells bind-ing to lgE aridanli IgE mAb- HRP were stairned with DAB plus neu-tral red. Results We found that only speeifie- sensitized mast cells was stained orange- brown colour. Conclusion This method can be used to forecast the development of type I allergy, such as asthma ete. and to conduct some expen ments on the degranulated mast cells.