牙龈卟啉单胞菌对脐静脉血管内皮细胞增殖的影响

Effect of Porphyromonas Gingivalis on Human Umbilical Vein Endothelial Cell Proliferation

目的 :观察牙龈卟啉单胞菌 (Pg)对人脐静脉血管内皮细胞 (HUVEC)增殖的影响 ,探讨Pg对牙龈血管内皮细胞的毒性作用。方法 :以厌氧袋法培养Pg ,原代培养HUVEC ,四甲基偶氮唑盐微量酶反应比色法 (MTT)观察HUVEC的增殖状态。结果 :活的和灭活的Pg都剂量依赖性地抑制HUVEC的增殖 ,活的Pg作用比灭活的Pg更为强烈 ,而Pg毒力株W83和非毒力株ATCC 332 77对HUVEC增殖的影响无显著差异。结论 :Pg可能通过损伤牙龈组织的血管内皮细胞加重局部的炎性反应 ,可能是Pg诱导牙周组织破坏的病理机制之一。

Objective: Our purpose was to observe the effect of porphyromonas gingivalis (Pg) on the proliferation of human umbilical vein endothelial cell (HUVEC) to investigate the virulence of Pg to vascular endothelial cells in gingiva. Methods: An aeropack was used to culture Pg, and HUVECs were derived from human umbilical vein. The MTT was employed to assay the proliferation of HUVEC. Results: Both viable Pg and deactivated one inhibited the proliferation of HUVEC in a dose-dependent manner. The inhibitive function of viable Pg was more than that of deactivated Pg. No significant difference was found between virulent strain W83 and avirulent strain ATCC 33277. Conclusion: The Pg might injure the endothelial cells in gingival tissue to aggravate the inflammatory reaction in periodontitis, which could be one of the pathologic mechanisms in periodontal disease.